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69744 pET-22b(+) DNA - Novagen

The Novagen pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag sequence.

pET-22b(+) DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69744
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69744-3 Plastová ampulka 10 μg
Description
OverviewThe pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins.
The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB038).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69744
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69744-3 07790788054403

Documentation

pET-22b(+) DNA - Novagen MSDS

Title

Safety Data Sheet (SDS) 

pET-22b(+) DNA - Novagen Certificates of Analysis

TitleLot Number
69744

Citations

Title
  • Arima, J., et al. 2008. Protein Engineering Design and Selection 21, 45.
  • Kimberly A. Burkhard and Angela Wilks. 2007. Journal of Biological Chemistry 282, 15126.
  • Cao, et al. W., 2007. Journal of Biological Chemistry 282, 18922.
  • Fujii, T., et al. 2007. Journal of Biochemistry (Tokyo) 141, 699.
  • Mark S. McClain and Timothy L. Cover. 2007. Infection and Immunity 75, 1785.
  • Nishiwaki, H., et al. 2007. Applied and Enviornmental Microbiology 73, 3404.
  • Roy, L.M., et al. 2007. Journal of Biological Chemistry 282, 15790.
  • Katja Strube, Simon de Vries and Rainer Cramm. 2007. Journal of Biological Chemistry 282, 20292.
  • Elizebeth C. Turner and Charles J. Dorman. 2007. Journal of Bacteriology 189, 3403.
  • Williams, E.P., et al. 2007. Journal of Bacteriology 189, 4234.
  • Chédin, F., et al. 2006. Journal of Biological Chemistry 281, 18610.
  • Langenheim, J.F., et al. 2006. Molecular Endocrinology 20, 661.
  • Carmelo, E., et al. 2005. Journal of Biological Chemistry 280, 28683.
  • Csizmok, V., et al. 2005. Molecular and Cellular Proteomics 5, 265.
  • Elia, N., et al. 2005. Journal of Cell Biology 171, 517.
  • Fierobe, H-P., et al. 2005. Journal of Biological Chemistry 280, 16325.
  • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
  • Loiseau, L., et al. 2005. Journal of Biological Chemistry 280, 26760.
  • Lucet, I.S., et al. 2005. Journal of Biological Chemistry 280, 2503.
  • Moore, J., et al. 2005. Journal of Biological Chemistry 280, 31489.
  • Oh, K.J., et al. 2005. Journal of Biological Chemistry 280, 753.
  • Amit V. Pandey and Walter L. Miller. 2005. Journal of Biological Chemistry 280, 13265.
  • Proctor, M.R., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 2697.
  • Varrot, A., et al. 2005. Journal of Biological Chemistry 280, 20181.
  • Williams, J.C., et al. 2005. Journal of Biological Chemistry 15202.
  • Yatsula, B., et al. 2005. Journal of Biological Chemistry 280, 30712.
  • Zhou, H., et al. 2005. Journal of Biological Chemistry 280, 13841.
  • Zhu, G., et al. 2005. Journal of Biological Chemistry 280, 23057.
  • Dong, T., et al. 2004. Journal of Experimental Medicine 200, 1547.
  • Hiroyasu Furukawa and Eric Gouaux. 2003. EMBO J. 22, 2873.
  • Seybert, A., et al. 2002. Nucleic Acids Research 30, 4329.
  • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. 2001. Procedings of the National Academy of Science 98, 445.
  • Jae Hyung Koo, Ick Hyun Cho and Yu Sam Kim. 2000. Journal of Bacteriology 182, 6382.
  • Z. Lin, T. Thorsen and F.H. Arnold. 1999. Biotechnol. Prog. 15, 467.
  • Gavin MacBeath, Peter Kast and Donald Hilvert. 1998. Science 279, 1958.
  • MacKenzie-Graham, A.J., et al. 1997. Journal of Immunology 159, 4601.
  • Ralph H. Lambalot and Christopher T. Walsh. 1995. Journal of Biological Chemistry 270, 24658.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB038VM pET-22b(+) Vector Map

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    Categories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors