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69436 pET-11a DNA - Novagen

Novagen's pET-11a-d vectors carry an N-terminal T7-Tag sequence and BamH I cloning site. The pET-21a-d(+) series corresponds to pET-11a-d but incorporates several additional features.

pET-11a DNA - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69436
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69436-3 Plastová ampulka 10 μg
Description
OverviewThe pET-11a-d vectors carry an N-terminal T7•Tag® sequence and BamH I cloning site. These vectors are the precursors to many pET family vectors; the pET-21a-d(+) series corresponds to pET-11a-d but incorporates several additional features. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map (TB042).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69436
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69436-3 04055977274295

Documentation

pET-11a DNA - Novagen Certificates of Analysis

TitleLot Number
69436

Citations

Title
  • Corinne D. Hausmann, Mette Prætorius-Ibba and Michael Ibba. (2007) An aminoacyl-tRNA synthetase:elongation factor complex for substrate channeling in archaeal translation. Nucleic Acids Research 35, 6094-6102.
  • James B. Ames, et al. (2006) Structural basis for calcium-induced inhibition of rhodopsin kinase by recoverin. Journal of Biological Chemistry 281, 37237-37245.
  • Wonduck Kim and F. Robert Tabita. (2006) Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. Journal of Bacteriology 188, 6544-6552.
  • Anastasia Metlitskaya, et al. (2006) Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic microcin C. Journal of Biological Chemistry 281, 18033-18042.
  • Hiroshi M. Sasaki, et al. (2006) Structural and mutational studies of the amino acid-editing domain from archaeal/eukaryal phenylalanyl-tRNA synthetase. Procedings of the National Academy of Science 103, 14744-14749.
  • Ryan Simkovsky and Jonathan King. (2006) An elongated spine of buried core residues necessary for in vivo folding of the parallel β-helix of P22 tailspike adhesin. Proceedings of the National Academy of Sciences (USA) 103, 3575-3580.
  • Irina Bruck, Roxana E. Georgescu and Mike O'Donnell. (2005) Conserved interactions in the Staphylococcus aureus DNA polC chromosome replication machine. Journal of Biological Chemistry 280, 18152-18162.
  • Giulia Calloni, et al. (2005) Investigating the effects of mutations on protein aggregation in the cell. Journal of Biological Chemistry 280, 10607-10613.
  • Jessica C. Greene, et al. (2005) Genetic and genomic studies of Drosophila parkin mutants implicate oxidative stress and innate immune responses in pathogenesis. Human Molecular Genetics 14, 799-811.
  • Noriko Handa, et al. (2005) Crystal structure of a novel polyisoprenoid-binding protein from Thermus thermophilus HB8. Protein Science 14, 1004-1010.
  • Valerie Heurgue-Hamard, et al. (2005) The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 Is methylated by the product of the YDR140w gene. Journal of Biological Chemistry 280, 2439-2445.
  • Frank Hillger, et al. (2005) Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. Journal of Biological Chemistry 280, 14974-14980.
  • Mette Praetorius Ibba, et al. (2005) Association between archaeal prolyl-and leucyl-tRNA synthetases enhances tRNAPro aminoacylation. Journal of Biological Chemistry 280, 26099-26104.
  • Eva Johansson, et al. (2005) Structures of dCTP deaminase from Escherichia coli with bound substrate and product: reaction mechanism and determinants of mono- and bifunctionality for a family of enzymes. Journal of Biological Chemistry 280, 3051-3059.
  • Peter A. Lemaire, Jeffrey Lary and James L. Cole. (2005) Mechanism of PKR activation: dimerization and kinase activation in the absence of double-stranded RNA. Journal of Molecular Biology 345, 81-90.
  • Mette Praetorius-Ibba, et al. (2005) Association between archaeal prolyl- and leucyl-tRNA synthetases enhances tRNApro aminoacylation. Journal of Biological Chemistry 280, 26099-26104.
  • Rongde Qiu, et al. (2005) Identification of the putative staphylococcal AgrB catalytic residues involving in the proteolytic cleavage of AgrD to generate autoinducing peptide. Journal of Biological Chemistry 280, 16695-16704.
  • Ok Hee Ryu, et al. (2005) Proteolysis of MIP-1α isoforms LD78β and LD78α by neutrophil-derived serine proteases. Journal of Biological Chemistry 280, 17415-17421.
  • D. Andrew Skaff, et al. (2005) Glucose 6-phosphate release of wild-type and mutant human brain hexokinases from mitochondria. Journal of Biological Chemistry 280, 38403-38409.
  • Wayne W. Chan, Steven L. Roderick and David E. Cohen. (2002) Human phosphatidylcholine transfer protein: purification, crystallization and preliminary X-ray diffraction data. Biochimica et Biophysica Acta 1596, 1-5.
  • Angelina J. Lay, et al. (2000) Phosphoglycerate kinase acts in tumor angiogenesis as a disulphide reductase. 408, 869-873.
  • Trudy H. Grossman, et al. (1998) Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene 209, 95-103.
  • K.M. Bohren, et al. (1991) Expression of human aldose and aldehyde erductases. Journal of Biological Chemistry 266, 24031-24037.
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    User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB042VM pET-11a-d Vector Map

    Vector Sequence

    Title
    pET-11a Vector Sequence