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559324 p38α, His•Tag®, Human, Recombinant, E. coli

p38α, His•Tag®, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: p38α, CSBP, Mxi2, p38, RK, SAPK2α

559324
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human p38α/SAPK2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified SAP Kinase in vitro with MKK6. Suitable for labeling SAP Kinase substrates.
Catalogue Number559324
Brand Family Calbiochem®
Synonymsp38α, CSBP, Mxi2, p38, RK, SAPK2α
References
ReferencesGuo, X., et al. 2003. J. Biol. Chem. 278, 22237.
Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol of phosphate to myelin basic protein (MBP) per min at 30°C.
FormLiquid
FormulationIn 150 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥12,000 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsProteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
559324 0

Documentation

p38α, His•Tag®, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

p38α, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
559324

References

Reference overview
Guo, X., et al. 2003. J. Biol. Chem. 278, 22237.
Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-September-2008 RFH
Synonymsp38α, CSBP, Mxi2, p38, RK, SAPK2α
DescriptionRecombinant, human p38α/SAPK2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified SAPK2α in vitro with MKK6. Suitable for labeling SAPK2α substrates.
FormLiquid
FormulationIn 150 mM NaCl, 25 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions20 mM Tris-HCl or MOPS, 18.75 mM MgCl2, 25 mM β-glycerolphosphate, 5 mM EGTA, 1 mM sodium orthovanadate, 1 mM DTT, 0.5 mg/ml myelin basic protein, 125 µM ATP (0.0375 µCi/µl), pH 7.5, 30°C.
Purity≥95% by SDS-PAGE
ContaminantsProteases: none detected
Specific activity≥12,000 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol of phosphate to myelin basic protein (MBP) per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesGuo, X., et al. 2003. J. Biol. Chem. 278, 22237.
Sanz-Moreno V., et al. 2003. Mol. Cell Biol. 23, 3079.