Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

109215 Ziehl-Neelsenov roztok karbolfuchsínu

for microscopy

Ziehl-Neelsenov roztok karbolfuchsínu MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Carbol fuchsin solution

109215
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092150100 Sklená flaša 100 ml
1092150500 Plastová flaša 500 ml
1092150507 Plastová flaša 500 ml
1092152500 Plastová flaša 2.5 l
Description
Catalogue Number109215
SynonymsCarbol fuchsin solution
DescriptionZiehl-Neelsen carbol-fuchsin solution
OverviewZiehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB). The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Density0.99 g/cm3 (20 °C)
Flash point47 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H226: Horľavá kvapalina a pary.
H314: Spôsobuje vážne poleptanie kože a poškodenie očí.
H341: Podozrenie, že spôsobuje genetické poškodenie.
H410: Veľmi toxický pre vodné organizmy, s dlhodobými účinkami.
Precautionary Statement(s)P210: Uchovávajte mimo dosahu tepla, horúcich povrchov, iskier, otvoreného ohňa a iných zdrojov zapálenia. Nefajčite.
P273: Zabráňte uvoľneniu do životného prostredia.
P280: Použite ochranné rukavice/ ochranný odev/ ochranu očí/ ochranu tváre/ ochranu sluchu.
P303 + P361 + P353: PRI KONTAKTE S POKOŽKOU (alebo vlasmi): Vyzlečte všetky kontaminované časti odevu. Pokožku ihneď opláchnite vodou.
P304 + P340 + P310: PO VDÝCHNUTÍ: Presuňte osobu na čerstvý vzduch a umožnite jej pohodlne dýchať. Okamžite volajte TOXIKOLOGICKÉ INFORMAČNÉ CENTRUM/lekára.
P305 + P351 + P338: PO ZASIAHNUTÍ OČÍ: Niekoľko minút ich opatrne vyplachujte vodou. Ak používate kontaktné šošovky a je to možné, odstráňte ich. Pokračujte vo vyplachovaní.
Signal WordNebezpečenstvo
Storage class3 Vznietlivé kvapaliny
WGKWGK 3 silne ohrozujúce vody
Disposal9
Karcinogénne zlúčeniny a horľavé zlúčeniny označené ako "veľmi jedovaté" alebo "jedovaté": nádoba F. Alkylsulfáty sú karcinogénne. Buďte osobitne opatrní, aby nedošlo k vdýchnutiu alebo ku kontaktu s pokožkou. Alkylsulfáty neutralizujte pridaním (z oddeľovacieho lievika) koncentrovaného ľadového roztoku amoniaku (kat. č. 105426) po kvapkách za intenzívneho miešania. Pred vyliatím do nádoby D skontrolujte pH pomocou indikátorových prúžkov pH-Universal (kat. č. 109535).
Safety Information
Categories of dangerhor?avá, škodlivá, žieravá, mutagénna
Product Usage Statements
Storage and Shipping Information
StorageSkladujte pri +15°C až +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1992 , 3 (6.1), III
Declaration (transport by air) IATA-DGRUN 1992 , 3 (6.1), III
Declaration (transport by sea) IMDG-CodeUN 1992 , 3 (6.1), III
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
1092150100 04022536112008
1092150500 04022536112015
1092150507 04054839116780
1092152500 04022536112039

Documentation

Ziehl-Neelsenov roztok karbolfuchsínu MSDS

Title

Safety Data Sheet (SDS) 

Ziehl-Neelsenov roztok karbolfuchsínu Certificates of Analysis

Product Number PackagingSpecificationLot Number
1092150100 Sklená flašaPDF Specification Document
1092150500 Plastová flašaPDF Specification Document
1092150507 Plastová flašaPDF Specification Document
1092152500 Plastová flašaPDF Specification Document

User Guides

Title
User Guide (IFU) - 109215

Related Products & Applications

Alternative Products

Catalogue Number Description
100497 AFB-color, modifikovaný

Accessories (Primary)

Catalogue Number Description
100327 Kyselina chlorovodíková v etanole
101287 Löfflerov roztok metylénovej modrej
116450 AFB-Color, farbiaca súprava

Accessories (Secondary)

Catalogue Number Description
100497 AFB-color, modifikovaný

Related Products By: Application Facete

Bacteria Staining

Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Auxiliaries for Bacteriology
IVD/OEM Materials & Reagents
IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions
IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining