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2910 Whole Cell Extraction Kit

Whole Cell Extraction Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Extraction
2910
100 assays  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number2910
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionWhole Cell Extraction Kit
Materials Required but Not Delivered1. Cultured Cells and Tissue Culture Supplies

2. Cell Detachment Buffer (Trypsin) or Cell Scrapers

3. Phosphatase Inhibitors (if desired)

4. Syringes, 1mL - 27 Gauge Needle

5. 1.5 mL Microcentrifuge tubes

6. Deionized Water

7. Table-top Microcentrifuge, 4oC

8. Phosphate-Buffered Saline (PBS), Tris-Buffered Saline (TBS), or HBSS (Hank's Balanced Salt Solution)
Product Information
Components
  • Whole Cell Extraction Buffer, 5x: - (Part No. 90493) Two vials containing 10 mL of 5x concentrated buffer. Dilute to 1x final concentration with ddH2O prior to use.
  • Protease Inhibitor Cocktail: - (Part No. 90492) One vial containing 100μL of protease inhibitors in DMSO for use with mammalian cells and tissue extracts. A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, and aspartic acid proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators. Prior to use, dilute 1/1000 in 1x Whole Cell Extraction Buffer.
Applications
Key Applications
  • Protein Extraction
Application NotesCHEMICON®'s Whole Cell Extraction Kit (Catalog No. 2910) provides a simple and convenient method for the preparation of whole cell extractions for use in experimental studies of various cellular proteins.

This kit is recommended for use with CHEMICON®'s Rac Activation Assay Kit (Catalog No. SGT425), Cdc42 Activation Assay Kit (Catalog No. SGT430), Ras Activation Assay Kit (Catalog No. SGT435), and CHEMICON®'s Transcription Factor Assays when purified nuclear extracts are not required.

For Research Use Only; Not for use in diagnostic procedures
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions· The 5x Whole Cell Extraction Buffer can be stored at 2-8°C until expiration date.

· The prepared 1x Whole Cell Extraction Buffer (with optional additives) should be stored at -20°C for up to one month.

· The undiluted Protease Inhibitor Cocktail can be stored at -20°C until the expiration date.
Packaging Information
Material Size100 assays
Global Trade Item Number
Catalogue Number GTIN
2910 04053252471995