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676472 VEGF₁₆₅, Human, Recombinant, S. frugiperda

VEGF₁₆₅, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Vascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin

676472
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, human VEGF165 (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.
Catalogue Number676472
Brand Family Calbiochem®
SynonymsVascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
References
ReferencesLiu, X., et al. 2001. J. Urology 166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg VEGF
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
676472 0

Documentation

VEGF₁₆₅, Human, Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

VEGF₁₆₅, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
676472

References

Reference overview
Liu, X., et al. 2001. J. Urology 166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.

Citations

Title
  • Diane Gesty-Palmer, et al. (2005) b-Arrestin 2 expression determines the transcriptional response to lysophosphatidic acid stimulation in murine embryo fibroblasts. Journal of Biological Chemistry 280, 32157-32167.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision16-September-2008 RFH
    SynonymsVascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
    DescriptionRecombinant, human VEGF165 (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfied-linked homodimer.
    FormLyophilized
    FormulationLyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.
    Purity≥97% by SDS-PAGE
    ContaminantsEndotoxin: ≤1 EU/µg VEGF
    Biological activityED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells
    SolubilityReconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
    Storage ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.
    Toxicity Standard Handling
    ReferencesLiu, X., et al. 2001. J. Urology 166, 354.
    Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
    Neufeld, G., et al. 1994. Prog. Growth Factor Res. 5, 89.
    Leung, D.W., et al. 1989. Science 246, 1306.
    Citation
  • Diane Gesty-Palmer, et al. (2005) b-Arrestin 2 expression determines the transcriptional response to lysophosphatidic acid stimulation in murine embryo fibroblasts. Journal of Biological Chemistry 280, 32157-32167.
    		•