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662060 Ubiquitin, His•Tag®, Recombinant, E. coli

662060
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant ubiquitin fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for ubiquitination of protein substrates and subsequent metal chelate affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using 6-His-specific antibodies.
Catalogue Number662060
Brand Family Calbiochem®
Synonyms(His)6-Ub
References
ReferencesBeers, E.P., and Callis, J. 1993. J. Biol. Chem. 268, 21645.
Product Information
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
662060 0

Documentation

Ubiquitin, His•Tag®, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Ubiquitin, His•Tag®, Recombinant, E. coli Certificates of Analysis

TitleLot Number
662060

References

Reference overview
Beers, E.P., and Callis, J. 1993. J. Biol. Chem. 268, 21645.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Proteasomes Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-July-2008 JSW
Synonyms(His)6-Ub
DescriptionRecombinant ubiquitin fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for metal chelate affinity purification of ubiquitinated molecules. Can also be used for immunodetection of conjugates using 6-His-specific antibodies.
FormLyophilized
Purity≥95% by SDS-PAGE
SolubilityAqueous buffers or DMSO
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
Toxicity Standard Handling
ReferencesBeers, E.P., and Callis, J. 1993. J. Biol. Chem. 268, 21645.