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662092 Ubiquitin Conjugating Enzyme 5b, GST-Fusion, Human, Recombinant, E. coli

662092
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human ubiquitin conjugating enzyme 5b fused to GST and expressed in E. coli. E2s are a family of ubiquitin carrier proteins that transfer ubiquitin to the ε-lysine of proteins designated for degradation. UbcH5b is a human homolog of the yeast UBC4/5 family which has been shown to play many important regulatory roles in inflammation and cancer.
Catalogue Number662092
Brand Family Calbiochem®
SynonymsGST-UbcH5b (E2)
References
ReferencesVarshavsky, A. 1997. Trends Biochem. Sci. 22, 383.
Hochstrasser, M. 1996. Annu. Rev. Genet. 30, 405.
Jensen, J.P., et al. 1995. J. Biol. Chem. 270, 30408.
Ciechanover, A. 1994. Cell 79, 13.
Seufert, W., and Jentsch, S. 1990. EMBO J. 9, 543.
Product Information
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10% glycerol, pH 8.0.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
662092 0

Documentation

Ubiquitin Conjugating Enzyme 5b, GST-Fusion, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
662092

References

Reference overview
Varshavsky, A. 1997. Trends Biochem. Sci. 22, 383.
Hochstrasser, M. 1996. Annu. Rev. Genet. 30, 405.
Jensen, J.P., et al. 1995. J. Biol. Chem. 270, 30408.
Ciechanover, A. 1994. Cell 79, 13.
Seufert, W., and Jentsch, S. 1990. EMBO J. 9, 543.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
SynonymsGST-UbcH5b (E2)
DescriptionRecombinant, human ubiquitin conjugating enzyme 5b fused to GST and expressed in E. coli. E2s are a family of ubiquitin carrier proteins that transfer ubiquitin to the ε-lysine of proteins designated for degradation. Forms thiol ester adducts with ubiquitin and has an activity similar to UbcH5a. UbcH5b is a human homolog of the yeast UBC4/5 family that has been shown to play important regulatory roles in inflammation and cancer.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesVarshavsky, A. 1997. Trends Biochem. Sci. 22, 383.
Hochstrasser, M. 1996. Annu. Rev. Genet. 30, 405.
Jensen, J.P., et al. 1995. J. Biol. Chem. 270, 30408.
Ciechanover, A. 1994. Cell 79, 13.
Seufert, W., and Jentsch, S. 1990. EMBO J. 9, 543.