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662098 UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli

UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: UBD2D3, Ubiquitin-conjugating enzyme E2 D3

662098
  
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Overview

Replacement Information
Description
OverviewFull-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
Catalogue Number662098
Brand Family Calbiochem®
SynonymsUBD2D3, Ubiquitin-conjugating enzyme E2 D3
References
ReferencesBrzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
Gehrke, S.G., et al. 2003 Blood. 101, 3288.
Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.
Product Information
FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
662098 0

Documentation

UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

UbcH5c Conjugating Enzyme E2, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
662098

References

Reference overview
Brzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
Gehrke, S.G., et al. 2003 Blood. 101, 3288.
Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-May-2009 JSW
SynonymsUBD2D3, Ubiquitin-conjugating enzyme E2 D3
DescriptionFull-length, recombinant, human UbcH5c expressed in E. coli. UbcH5c, an E2 ubiquitin ligase, is highly homologous to the yeast UbC4/5 family of E2 enzymes. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of transcription factors such as p53, NF-κB3, and c-Fos. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
FormulationIn 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditionsTypical concentration for conjugate formation: 100 nM to 1 µM depending on the conditions
Purity≥95% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesBrzovic, P.S., et al. 2006. Cell Cycle 5, 2867.
Gehrke, S.G., et al. 2003 Blood. 101, 3288.
Nuber, U., And Scheffner, M., 1999. J. Biol. Chem. 274, 7576.
Schwarz, S.E., et al. 1998. J. Biol. Chem. 273, 12148.
Jensen, J., et al. 1995. J. Biol. Chem. 270, 30408.
Scheffner, M., et al. 1994. Proc. Natl. Acad. Sci. 91, 8797.