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616377 Toxin B, Clostridium difficile - Calbiochem

Toxin B, Clostridium difficile - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Cytotoxin

616377
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
Catalogue Number616377
Brand Family Calbiochem®
SynonymsCytotoxin
References
ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
Primary TargetSmall GTPases, such as Rho, Rac, and Cdc42
Puritymajor band at >200 kDa
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSXW5807300
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 36-26

Wear suitable protective clothing.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616377 0

Documentation

Toxin B, Clostridium difficile - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Toxin B, Clostridium difficile - Calbiochem Certificates of Analysis

TitleLot Number
616377

References

Reference overview
Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
SynonymsCytotoxin
DescriptionA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
FormLyophilized
FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
RTECSXW5807300
Puritymajor band at >200 kDa
Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
SolubilitySterile dH₂O. Please see vial label for lot-specific reconstitution volume.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
Toxicity Irritant
ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.