Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
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-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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605195
Sigma-AldrichThrombin, Human Plasma, High Activity
Designed for use in thrombin clotting time tests. Key enzyme that activates factor XIII and converts fibrinogen into fibrin.
Catalogue Number
605195
Brand Family
Calbiochem®
References
References
Forsberg, G., et al. 1992. J. Protein Chem.11, 201. Garcia, J.G. 1992. J. Lab. Clin. Med.120, 513. Scarborough, R.M., et al. 1992. J. Biol. Chem.267, 13146. Weigel, L.M., et al. 1992. Infect. Immun.60, 1568.
Product Information
CAS number
9002-04-4
Unit of Definition
One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.
Form
Lyophilized solid
Formulation
Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.
Thrombin, Human Plasma, High Activity Certificates of Analysis
Title
Lot Number
605195
References
Reference overview
Forsberg, G., et al. 1992. J. Protein Chem.11, 201. Garcia, J.G. 1992. J. Lab. Clin. Med.120, 513. Scarborough, R.M., et al. 1992. J. Biol. Chem.267, 13146. Weigel, L.M., et al. 1992. Infect. Immun.60, 1568.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-May-2008 RFH
Description
Designed for use in thrombin clotting time tests. Serine protease formed in the plasma during the clotting process from prothrombin, calcium, and thromboplasmin. Acts on the soluble plasma protein fibrinogen to convert it to an insoluble fibrin clot. Prepared from homogenous human prothrombin by activation with Factor Xa, Factor Va, and phospholipid. Designed for use in thrombin time tests.
Form
Lyophilized solid
Formulation
Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 0.1% PEG-8000, pH 6.5.
Source
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number
9002-04-4
RTECS
XO8950000
Purity
Complete activation from homogeneous prothrombin by SDS-PAGE
Specific activity
≥2800 NIH units/mg protein
Unit definition
One unit is determined by comparison with a standard curve prepared using the Bureau of Biologics standard thrombin.
Solubility
H₂O (1 mg/ml). Solution will have a cloudy appearance.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Harmful
Merck USA index
14, 9383
References
Forsberg, G., et al. 1992. J. Protein Chem.11, 201. Garcia, J.G. 1992. J. Lab. Clin. Med.120, 513. Scarborough, R.M., et al. 1992. J. Biol. Chem.267, 13146. Weigel, L.M., et al. 1992. Infect. Immun.60, 1568.
