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58346 Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem

CAS 64-17-5 is a broad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.

Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: binding of aminoacyl tRNA to the A-site of ribosomes
58346
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
64-75-5C₂₂H₂₄N₂O₈
Description
OverviewBroad-based antibiotic agent that blocks protein synthesis by inhibiting binding of aminoacyl tRNA to the A-site of ribosomes. Induces cold shock-response and enhances P450 expression in bacteria.
Catalogue Number58346
Brand Family Calbiochem®
References
ReferencesKusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.
Product Information
CAS number64-75-5
Activity≥900 µg/mg
FormYellow crystalline solid
Hill FormulaC₂₂H₂₄N₂O₈
Chemical formulaC₂₂H₂₄N₂O₈
Structure formula ImageStructure formula Image
Quality LevelMQ200
Applications
Biological Information
Primary Targetbinding of aminoacyl tRNA to the A-site of ribosomes
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSQI9100000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
58346 0

Documentation

Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Tetracycline, Hydrochloride - CAS 64-75-5 - Calbiochem Certificates of Analysis

TitleLot Number
58346

References

Reference overview
Kusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.

Brochure

Title
Antibiotics Profiler
Overnight Express Autoinduction Systems

Citations

Title
  • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision13-September-2007 JSW
    DescriptionAn antibiotic that inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit, preventing binding of aminoacyl tRNA to the A-site and blocking translocation. Used commonly for selection of the bacterial tetr gene whose product, a 399-amino acid membrane-associated protein, functions in active export of the drug from the cell.
    FormYellow crystalline solid
    CAS number64-75-5
    RTECSQI9100000
    Chemical formulaC₂₂H₂₄N₂O₈
    Structure formulaStructure formula
    Activity≥900 µg/mg
    SolubilityEthanol (5 mg/ml) or H₂O (20 mg/ml)
    Storage Protect from moisture
    Protect from light
    +15°C to +30°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for 1 month at -20°C. Agar plates are stable for 2 weeks at 4°C. Autoclaved media should be cooled to ≤55°C before adding tetracycline.
    Toxicity Standard Handling
    Merck USA index14, 9196
    ReferencesKusano, K., et al. 1999. Arch. Biochem. Biophys. 367, 129.
    Lech, K. and Brent, R. 1995. In Current Protocols in Molecular Biology, Vol. 1 (Ausubel, F.M., et al. eds.) John Wiley & Sons, New York Unit 1.4.
    Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York, p. 1.6, A.6.
    Chopra, I. and Howe, T.G.B. 1978. Microbiol. Rev. 42, 707.
    Citation
  • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
    		•