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654205 TNF-α, Human, Recombinant, E. coli

654205
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Overview

Products

Catalogue NumberPackaging Qty/Pack
654205-10UG Plastová ampulka 10 μg
Description
OverviewRecombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number654205
Brand Family Calbiochem®
SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
ReferencesMerck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
Product Information
CAS number94948-59-1
FormLyophilized
FormulationCarrier-free
Quality LevelMQ100
Biological Information
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
Safety Information according to GHS
RTECSMT6435000
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Global Trade Item Number
Catalogue Number GTIN
654205-10UG 04055977184594