Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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654205
Sigma-AldrichTNF-α, Human, Recombinant, E. coli
Recombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number
654205
Brand Family
Calbiochem®
Synonyms
rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
References
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity
≥95% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤0.1 ng/µg TNF-α
Safety Information according to GHS
RTECS
MT6435000
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
TNF-α, Human, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
654205
References
Reference overview
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-January-2009 JSW
Synonyms
rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
Description
Recombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
Form
Lyophilized
Formulation
Carrier-free
CAS number
94948-59-1
RTECS
MT6435000
Purity
≥95% by SDS-PAGE
Contaminants
Endotoxin: ≤0.1 ng/µg TNF-α
Biological activity
ED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Solubility
Reconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Toxicity
Standard Handling
References
Merck Index 13, 9882. Tsuchida, H., et al. 1995. J. Immunol. 154, 2403. Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689. Polunovsky, V.A., et al. 1994. Exp. Cell Res.214, 584. Koide, M., et al. 1993. FEBS Lett.318, 213. Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem.266, 7313.
