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14-748M Src (T341M) Protein, active, 250 µg

Active, N-terminal 6His-tagged, recombinant, full-length human Src, containing the T341M mutation. For use in Kinase Assays.

Src (T341M) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-748M
250 µg  Also available in 10 μg size (cat#14-748) and in bulk (cat# 14-748-K).
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-748M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSrc (T341M) Protein, active, 250 µg
OverviewN-terminal 6His-tagged, recombinant, full-length human Src, containing the T341M mutation.
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, full-length human Src, containing the T341M mutation. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • SRC
  • c-src
  • p60-Src
  • SRC1
  • c-SRC
  • ASV
  • pp60c-src
Protein TargetSrc
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummarySIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight61.7 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C from date of shipment.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-748) and in bulk (cat# 14-748-K).
Global Trade Item Number
Catalogue Number GTIN
14-748M 04053252478857