Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

530648 SIRT2 Inhibitor, AEM2 - Calbiochem

A cell permeable, selective inhibitor of SIRT2 (IC₅₀ = 3.8 µM).

SIRT2 Inhibitor, AEM2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sirtuin 2 Inhibitor

Primary Target: SIRT2
530648
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₇H₂₇N₃O
Description
Overview

This product has been discontinued.



A cell permeable methanophenanthridine derivative that acts a selective inhibitor of SIRT2 (IC50 = 3.8 µM) and exhibits ~30 fold greater selectivity over SIRT1 (IC50 > 100 µM) and SIRT3. Blocks SIRT2-induced p53 deacetylation and thereby elevates p53 levels and causes increased expression of CDKN1A, PUMA, and NOXA in non-small cell lung cancer (NSCLC) A549 cells. Also shown to reduce NSCLC cell viability by 50% (~20 µM) and inhibit their proliferation. Sensitizes A549 cancer cell line to etoposide (~1 µM) induced DNA damage in a p53-dependent manner.

Please note that the molecular weight for this compound is batch-specific due to variable water content.

Catalogue Number530648
Brand Family Calbiochem®
SynonymsSirtuin 2 Inhibitor
References
ReferencesHoffmann G., et al. 2014. J. Biol. Chem. 289, 5208.
Product Information
FormWhite solid
Hill FormulaC₂₇H₂₇N₃O
Chemical formulaC₂₇H₂₇N₃O
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetSIRT2
Primary Target IC<sub>50</sub>3.8 µM
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
530648 0

Documentation

SIRT2 Inhibitor, AEM2 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Hoffmann G., et al. 2014. J. Biol. Chem. 289, 5208.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-June-2014 JSW
SynonymsSirtuin 2 Inhibitor
DescriptionA cell permeable methanophenanthridine derivative that acts a selective inhibitor of SIRT2 (IC50 = 3.8 µM) and exhibits ~30 fold greater selectivity over SIRT1 (IC50 > 100 µM) and SIRT3. Blocks SIRT2-induced p53 deacetylation and thereby elevates p53 levels and causes increased expression of CDKN1A, PUMA, and NOXA in non-small cell lung cancer (NSCLC) A549 cells. Also shown to reduce NSCLC cell viability by 50% (~20 µM) and inhibit their proliferation. Sensitizes A549 cancer cell line to etoposide (~1 µM) induced DNA damage in a p53-dependent manner.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₇H₂₇N₃O
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml). Use only fresh DMSO for reconstitution.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C.
Toxicity Standard Handling
ReferencesHoffmann G., et al. 2014. J. Biol. Chem. 289, 5208.