Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

HTS032RTA Ready-to-Assay™ KiSS1 KiSS1-Derived Peptide Receptor Frozen Cells

Human KiSS1 / GPR54 GPCR frozen cells for Calcium Flux FLIPR Assays.

Ready-to-Assay™ KiSS1 KiSS1-Derived Peptide Receptor Frozen Cells MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_032551
HTS032RTA
1 kit  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

This product requires a User Agreement (Label License). Please see product datasheet for further information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1KiSS1 / GPR54
Description
Catalogue NumberHTS032RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ KiSS1 KiSS1-Derived Peptide Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

KiSS1 (also known as GPR54 and AXOR12) is a Gq-coupled GPCR that binds to a series of peptides, termed kisspeptins or metastin, derived from the KiSS-1 gene (Kotani et al., 2001; Muir et al., 2001). The KiSS-1 gene inhibits metastatic activity of melanoma and other tumor cell lines, and clinical evidence supports a role for KiSS-1 and GPR54 in inhibition of metastasis in human cancer (Harms et al., 2003). Kisspeptins and GPR54 also play a central role in hypothalamic regulation of puberty, by directly governing the release of gonadotropin-releasing hormone from the hypothalamus (Messager et al., 2005; Shahab et al., 2005). In addition, mutations in GPR54 in mice and humans result in hypogonadotropic hypogonadism (Colledge, 2004; Seminara et al., 2004). Millipore’s cloned human KiSS1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant KiSS1 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at KiSS1.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ100
Applications
ApplicationHuman KiSS1 / GPR54 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetKiSS1 / GPR54
Target Sub-FamilyKiSS1-derived peptide
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS032RTA 04053252377891

Documentation

Ready-to-Assay™ KiSS1 KiSS1-Derived Peptide Receptor Frozen Cells MSDS

Title

Safety Data Sheet (SDS) 

Related Products & Applications

Product Families

ChemiSCREEN™ GPCR Stable Cell Lines, Membranes Preparation and Ready-to-Assay™ Frozen Cells

Millipore offers a large selection of robust and reliable G-protein coupled receptor products, including Stable Cell Lines, Membrane Preparations, and Frozen Cells. See below for GPCR research tools.
Learn More >>

Related Products By: Brand Facete

Ready-to-Assay™

Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells