342001 Sigma-AldrichProcathepsin K, Human, Recombinant, E. coli
Recombinant, human procathepsin K expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site.
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Overview
Products
| Catalogue Number | Packaging | Qty/Pack | |
|---|---|---|---|
| 342001-10UG | Plastová ampulka | 10 μg |
| Description | |
|---|---|
| Overview | Recombinant, human procathepsin K (amino acids 19-329) (GenBank target symbol = S79895, ACC No. P43235) expressed in E. coli with a methionine residue inserted at amino acid 18 to create a new N-terminal initiation site. Cathepsin K, a member of the papain superfamily of cysteine proteinases, plays an important role in osteoclast-mediated bone resorption and collagen degradation. Cathepsin K is synthesized as an inactive proenzyme that is converted to its mature, active form by proteolytic cleavage of the 99 amino acid propeptide domain. Inhibitors of cathepsin K include leupeptin (Cat. No. 108975) (IC50 = 70 nM), E-64 (Cat. No. 324890) (IC50 = 5 nM), and cystatin (Cat. No. 324891 or 324896). Requires activation prior to use. |
| If the activated enzyme is not used immediately, it is recommended to add methyl methanthiosulfonate (1 mM final concentration MMTS). | |
| Note: 1 mU = 1 milliunit. | |
| Catalogue Number | 342001 |
| Brand Family | Calbiochem® |
| Product Information | |
|---|---|
| Activity | ≥1000 mU/mg protein |
| Unit of Definition | One unit is defined as the amount of enzyme that will hydrolyze 1 µmole benzyloxycarbonyl-phenylalanine-arginine-7-amido-4-methylcoumarin per min at 37°C, pH 5.5. |
| EC number | 3.4.22.38 |
| Form | Liquid |
| Formulation | In 500 mM NaCl, 25 mM Tris, pH 8.0. |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Purity | ≥95% by SDS-PAGE |
| Concentration Label | Please refer to vial label for lot-specific concentration |
| Global Trade Item Number | |
|---|---|
| Catalogue Number | GTIN |
| 342001-10UG | 04055977194999 |