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676602 PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

This PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Vasodilator-stimulated Phosphoprotein, Activated

676602
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody
Description
OverviewRecognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
Catalogue Number676602
Brand Family Calbiochem®
SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
Application Data
Detection of human VASP, phosphorylated on Ser239, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer239) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesReinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.
Product Information
FormLyophilized
FormulationLyophilized from 2X PBS, PEG, sucrose.
Positive controlVanadate-treated A431 cells or 8-pCPT-treated platelets
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (0.05 µg/ml)
Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application CommentsDoes not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton® X-100 detergent, and 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
ImmunogenHuman
Clone16C2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
676602 0

Documentation

PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) Certificates of Analysis

TitleLot Number
676602

References

Reference overview
Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2007 RFH
SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
ApplicationELISA (0.05 µg/ml)
Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Data
Detection of human VASP, phosphorylated on Ser239, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer239) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
BackgroundVASP is a proline-rich substrate of PKA and PKG. It is involved in the enhancement of actin filament formation and has been shown to bind profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. VASP is expressed in most mammalian tissues, with particularly high expression in platelets.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
Clone16C2
IsotypeIgG₁
Specieshuman, mouse
Positive controlVanadate-treated A431 cells or 8-pCPT-treated platelets
FormLyophilized
FormulationLyophilized from 2X PBS, PEG, sucrose.
Preservative≤0.1% sodium azide
CommentsDoes not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton® X-100 detergent, and 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesReinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.