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676604 PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Vasodilator-stimulated Phosphoprotein, Activated

676604
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
676604-100UG Plastová ampulka 100 μg
Description
OverviewRecognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Catalogue Number676604
Brand Family Calbiochem®
SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
Application Data
Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
References
ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
Product Information
FormLyophilized
FormulationLyophilized from PBS, PEG, sucrose.
Positive controlVanadate-treated A431 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
ImmunogenHuman
Clone5C6
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
676604-100UG 04055977260564

Documentation

PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) Certificates of Analysis

TitleLot Number
676604

References

Reference overview
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-September-2009 JSW
SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
Immunocytochemistry (1-10 µg/ml)
Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
Application Data
Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
BackgroundVASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser157 in VASP can be used as a measure of PKA activity.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Clone5C6
IsotypeIgG₁
Specieshuman, mouse
Positive controlVanadate-treated A431 cells
FormLyophilized
FormulationLyophilized from PBS, PEG, sucrose.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.