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AP1064 PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

This PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

AP1064
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1064-50UG Plastová ampulka 50 μg
Description
Overview

This product has been discontinued.



Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells.
Catalogue NumberAP1064
Brand Family Calbiochem®
SynonymsAnti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb
Application Data
Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.

Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains.
References
ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
Negative controlHEK293 cells treated with dichloroacetate
Positive controlHEK293 cells
Preservative≤0.1% sodium azide
Quality LevelMQ200
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (0.5 µg/ml)
Immunocytochemistry (1 µg/ml)
Application CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
ImmunogenHuman
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1064-50UG 04055977227802

Documentation

PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb Certificates of Analysis

TitleLot Number
AP1064

References

Reference overview
Rardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-October-2011 JSW
SynonymsAnti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb
ApplicationImmunoblotting (0.5 µg/ml)
Immunocytochemistry (1 µg/ml)
Application Data
Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.

Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300.
BackgroundThe pyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC is mainly regulated by phosphorylation-dephosphorylation of three specific serine residues (232, 293, 300) on the E1α subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α
Specieshuman
Positive controlHEK293 cells
Negative controlHEK293 cells treated with dichloroacetate
FormLiquid
FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDue to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesRardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.