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109272 Papanicolaouov roztok 3b, polychromatický roztok EA 50

109272
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092720500 Plastová flaša 500 ml
1092721000 Plastová flaša 1 l
1092722500 Plastová flaša 2.5 l
Description
Catalogue Number109272
DescriptionPapanicolaou's solution 3b polychromatic solution EA 50
OverviewPapanicolaou’s solution 3b polychromatic solution EA 50 - for cytology, is a ready-to-use solution used for human-medical cell diagnosis and serve the purpose of the cytological investigation of sample material of human origin, for example, gynecological and clinico-cytological specimen materials. Papanicolaou’s technique is the most used staining procedure for cytological specimen is and is intended for the the staining of exfoliative cells in cytological specimens. In the first step, the cell nuclei are stained either progressively or regressively with a hematoxylin solution, (Hematoxylin solution modified acc.to Gill II, (Cat.no. 105175), Papanicolaou’s solution 1a Harris hematoxylin solution, (Cat.no. 109253) or Papanicolaou’s solution 1b Hematoxylin solution S, (Cat.no. 109254)). Nuclei are stained blue to dark violet. In the progressive hematoxylin staining method, staining is carried out to the endpoint, after which the slide is blued in tapwater. With the regressive method the material is over-stained and the excess of staining solution is removed by acid rinsing steps, followed by the bluing step. The structures of nuclei are more differentiated and better visible by the regressive method. The second staining step is cytoplasmic staining by orange staining solution (Papanicolaou’s solution 2b Orange II solution, Cat. no. 1106887 or Papanicolaou’s solution 2a Orange G solution (OG6), Cat. no. 106888), especially for demonstration of mature and keratinized cells. The target structures are stained orange in different intensities. In the third staining step is used the so-called polychrome solution, a mixture of eosin, light green SF and Bismarck brown, (Papanicolaou’s solution 3a polychromatic solution EA 31, (Cat.no 109271), Papanicolaou’s solution 3b polychromatic solution EA 50, (Cat.no. 109272), Papanicolaou’s solution 3c polychromatic solution EA 65(Cat.no. 109270), o Papanicolaou’s solution 3d polychromatic solution EA 65, (Cat.no.109269). The polychrome solution is used for demonstration of differentiation of squamous cells. Papanicolaou’s solution 3b polychromatic solution EA 50 gives a blue-green-pinkish staining result in clinical specimen material.
The 500 ml bottle provides 1500 - 2000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 00
Quality LevelMQ400
Applications
Applicationfor cytology
Biological Information
Physicochemical Information
Density0.82 g/cm3 (20 °C)
Flash point17 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Veľmi horľavá kvapalina a pary.
H302 + H312 + H332: Zdraviu škodlivý pri požití, styku s kožou alebo pri vdýchnutí.
H317: Môže vyvolať alergickú kožnú reakciu.
H319: Spôsobuje vážne podráždenie očí.
H370: Spôsobuje poškodenie orgánov.
Precautionary Statement(s)P210: Uchovávajte mimo dosahu tepla, horúcich povrchov, iskier, otvoreného ohňa a iných zdrojov zapálenia. Nefajčite.
P280: Použite ochranné rukavice/ ochranný odev/ ochranu očí/ ochranu tváre/ ochranu sluchu.
P301 + P312: PO POŽITÍ: Pri zdravotných problémoch volajte NÁRODNÉ TOXIKOLOGICKÉ INFORMAČNÉ CENTRUM/lekára.
P303 + P361 + P353: PRI KONTAKTE S POKOŽKOU (alebo vlasmi): Vyzlečte všetky kontaminované časti odevu. Pokožku ihneď opláchnite vodou.
P304 + P340 + P312: PO VDÝCHNUTÍ: Presuňte osobu na čerstvý vzduch a umožnite jej pohodlne dýchať. Pri zdravotných problémoch volajte NÁRODNÉ TOXIKOLOGICKÉ INFORMAČNÉ CENTRUM/lekára.
P308 + P311: PO expozícii alebo podozrení z nej: volajte TOXIKOLOGICKÉ INFORMAČNÉ CENTRUM/lekára.
P403 + P233: Uchovávajte na dobre vetranom mieste. Nádobu uchovávajte tesne uzavretú.
Signal WordNebezpečenstvo
Storage class3 Vznietlivé kvapaliny
WGKWGK 2 významne ohrozuje vodné zdroje
Disposal1
Silno kontaminované organické rozpúštadlá neobsahujúce halogény: nádoba A.
Safety Information
Categories of dangerve?mi hor?avá, jedovatá
Product Usage Statements
Storage and Shipping Information
StorageSkladujte pri +15°C až +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1987 , 3, II
Declaration (transport by air) IATA-DGRUN 1987 , 3, II
Declaration (transport by sea) IMDG-CodeUN 1987 , 3, II
Supplemental Information
Specifications
Suitability for microscopy (Vaginal smear)passes test
Nucleiblue to dark violet
Cyanophilic Cytoplasm (basophilic)blue-green
Eosinophilic Cytoplasm (acidophilic)pink
Global Trade Item Number
Catalogue Number GTIN
1092720500 04022536112886
1092721000 04022536676685
1092722500 04022536112909

Documentation

Papanicolaouov roztok 3b, polychromatický roztok EA 50 MSDS

Title

Safety Data Sheet (SDS) 

Papanicolaouov roztok 3b, polychromatický roztok EA 50 Certificates of Analysis

Product Number PackagingSpecificationLot Number
1092720500 Plastová flašaPDF Specification Document
1092721000 Plastová flašaPDF Specification Document
1092722500 Plastová flašaPDF Specification Document

User Guides

Title
User Guide (IFU) - 109272

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
106887 Papanicolaouov roztok 2b, oranž II, roztok
106888 Papanicolaouov roztok 2a, oranž G, roztok (OG 6)
109253 Papanicolaouov roztok 1a, Harrisov roztok hematoxylínu
109254 Papanicolaouov roztok 1b, roztok hematoxylínu S
109269 Papanicolaouov roztok 3d, polychromatický roztok EA 65
109271 Papanicolaouov roztok 3a, polychromatický roztok EA 31
109270 Papanicolaouov roztok 3c, polychromatický roztok EA 65

Accessories (Secondary)

Catalogue Number Description
106887 Papanicolaouov roztok 2b, oranž II, roztok
106888 Papanicolaouov roztok 2a, oranž G, roztok (OG 6)
109253 Papanicolaouov roztok 1a, Harrisov roztok hematoxylínu
109254 Papanicolaouov roztok 1b, roztok hematoxylínu S
109269 Papanicolaouov roztok 3d, polychromatický roztok EA 65
109271 Papanicolaouov roztok 3a, polychromatický roztok EA 31
109270 Papanicolaouov roztok 3c, polychromatický roztok EA 65

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Categories

IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents