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539734 PTP, Yersinia enterocolitica, Recombinant, E. coli

539734
  
Purchase on Sigma-Aldrich

Overview

Description
Overview

This product has been discontinued.



Recombinant, 51 kDa catalytic domain of the Yersinia protein tyrosine phosphatase containing a C235R mutation and expressed in E. coli. Suitable for dephosphorylation of phosphotyrosine residues in proteins.

Catalogue Number539734
Brand Family Calbiochem®
SynonymsYOP, Protein Tyrosine Phosphatase, Yersenia
References
ReferencesZhang, Z.Y., et al. 1992. J. Biol. Chem. 267, 23759.
Gordon, J.A. 1991. Methods Enzymol. 201, 477.
Guan, K. and Dixon, J.E. 1990. Science 249, 653.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 nmol of <i>p</i>NPP per min at 30°C, pH 7.2.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 5 mM DTT, 2 mM EDTA, 0.01% BRIJ® 35 Detergent, 50% glycerol, pH 7.0.
Quality LevelMQ100
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥50,000 units/mg
Physicochemical Information
ContaminantsProteases, serine/threonine phosphatases: none detected
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
539734 0