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539493 PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli

PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase

539493
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, rabbit, Mn2+-dependent catalytic subunit of the α-isoform of protein phosphatase 1 expressed in E. coli. Hydrolyzes the phosphate groups on serine and threonine residues and has also been known to hydrolyze phosphates on tyrosine residues. Assists in tobacco mosaic virus-mediated programmed cell death.
Catalogue Number539493
Brand Family Calbiochem®
SynonymsProtein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase
References
ReferencesDunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.
Product Information
Activity≥500 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per minute at 30°C, pH 7.0.
FormLiquid
FormulationIn 200 mM NaCl, 50 mM HEPES, 2.5 mM DTT, 1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.025% TWEEN®-20 Detergent, pH 7.0.
Applications
Biological Information
Specific Activity13,500 units/mg
Physicochemical Information
ContaminantsProtease: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°c).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539493 0

Documentation

PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli Certificates of Analysis

TitleLot Number
539493

References

Reference overview
Dunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2008 RFH
SynonymsProtein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase
DescriptionRecombinant, rabbit, Mn2+-dependent catalytic subunit of the α-isoform of protein phosphatase 1 expressed in E. coli. Hydrolyzes the phosphate groups on serine and threonine residues, and has also been known to hydrolyze phosphates on tyrosine residues. Assists in tobacco mosaic virus-mediated programmed cell death.
FormLiquid
FormulationIn 200 mM NaCl, 50 mM HEPES, 2.5 mM DTT, 1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.025% TWEEN®-20 Detergent, pH 7.0.
Recommended reaction conditions50 mM Tris-HCl, 5 mM DTT, 200 µM MnCl2, 100 µM EDTA, and 200 µg/ml BSA, pH 7.0. 50 mM caffeine is required if phosphorylase is the substrate.
ContaminantsProtease: none detected
Specific activity13,500 units/mg
Activity≥500 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per minute at 30°C, pH 7.0.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°c).
Toxicity Standard Handling
ReferencesDunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.