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505892 PKR Inhibitor III, 7DG CAS 26927-01-5 - Calbiochem

PKR Inhibitor III, 7DG CAS 26927-01-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Double-stranded RNA-dependent Protein Kinase Inhibitor III, Double-stranded RNA-activated Protein Kinase Inhibitor III, EIF2AK2 Inhibitor III, 7-Desacetoxy-6,7-dehydrogedunin

Primary Target: PKR
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
26927-01-5C₂₆H₃₀O₅
Description
OverviewA cell-permeable gedunin analog that directly targets PKR via reversible interaction inhibits PKR-mediated cellular events by disrupting PKR signaling complexes assembly, such as IκK complex and inflammasome/pyroptosome, resulting in effective blockage of LPS-induced IκBβ degradaion (20 µM) and protection against anthrax toxin LT-induced caspase-1 activation as well as subsequent pyroptosis (10 µM) in murine macrophage J774 cultures. PKR is also known to mediate LT-induced apoptosis via its kinase activity in LPS-primed Macrophages derived from C57BL/6 with a resistent NLRP1 allele, inhibitable by C16 (Cat. Nos. 527450 & 527451), but not by 7DG, while LT-induced pyroptosis in J774 cultures is insensitive to C16 treatment.
Catalogue Number505892
Brand Family Calbiochem®
SynonymsDouble-stranded RNA-dependent Protein Kinase Inhibitor III, Double-stranded RNA-activated Protein Kinase Inhibitor III, EIF2AK2 Inhibitor III, 7-Desacetoxy-6,7-dehydrogedunin
References
ReferencesHett, E.C., et al. 2013. Nat. Chem. Biol. 9, 398.
Product Information
CAS number26927-01-5
FormPale yellow solid
Hill FormulaC₂₆H₃₀O₅
Chemical formulaC₂₆H₃₀O₅
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetPKR
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
505892 0

Documentation

PKR Inhibitor III, 7DG CAS 26927-01-5 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Hett, E.C., et al. 2013. Nat. Chem. Biol. 9, 398.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-September-2013 JSW
SynonymsDouble-stranded RNA-dependent Protein Kinase Inhibitor III, Double-stranded RNA-activated Protein Kinase Inhibitor III, EIF2AK2 Inhibitor III, 7-Desacetoxy-6,7-dehydrogedunin
DescriptionA cell-permeable gedunin analog that directly targets PKR via reversible interaction without competing against ATP binding and inhibits PKR-mediated cellular events by disrupting PKR signaling complex assembly, such as IκK complex and inflammasome/pyroptosome, resulting in effective blockage of LPS-induced IκBβ degradaion (20 µM 7DG added 2 h prior to 10 ng/mL LPS) and protection against anthrax toxin LT-induced caspase-1 activation as well as subsequent pyroptosis (by 50% and 100%, respectively, at 5 and 10 µM; 2 h 7DG pretreatment prior to 6 h LT incubation) in J774A.1 BALB/c-derived murine macrophage J774 cultures. PKR is also known to mediate LT-induced apoptosis via its kinase activity in LPS-primed Macrophages derived from C57BL/6 with a resistent NLRP1 allele, inhibitable by C16 (Cat. Nos. 527450 & 527451), but not by 7DG, while LT-induced pyroptosis in J774 cultures is inhibited by 7DG, but insensitive to C16 treatment.
FormPale yellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number26927-01-5
Chemical formulaC₂₆H₃₀O₅
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (25 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Toxicity Standard Handling
ReferencesHett, E.C., et al. 2013. Nat. Chem. Biol. 9, 398.