Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

12-123 PKC Substrate Peptide

PKC Substrate Peptide primarily used in Kinase Assays.

PKC Substrate Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
12-123
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number12-123
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKC Substrate Peptide
References
Product Information
PresentationLyophilized powder
Quality LevelMQ100
Applications
ApplicationPKC Substrate Peptide primarily used in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Gene Symbol
  • PRKCA
  • PKC-A
  • PKCA
  • MGC129901
  • AAG6
  • MGC129900
  • PRKACA
  • PKC-alpha
Protein TargetPKC
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 672 amino acids; 76764 Da
SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated in an enzyme assay using PKC (14-115)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at 4°C
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-123 04053252509568

Documentation

PKC Substrate Peptide MSDS

Title

Safety Data Sheet (SDS) 

PKC Substrate Peptide Certificates of Analysis

TitleLot Number
PKC Substrate Peptide (MBP 4-14) - 2378754 2378754
PKC Substrate Peptide (MBP 4-14) 2934604
PKC Substrate Peptide (MBP 4-14) - 2262762 2262762
PKC Substrate Peptide (MBP 4-14) - 3683138 3683138
PKC Substrate Peptide (MBP 4-14) -2452677 2452677
PKC Substrate Peptide - 15157 15157
PKC Substrate Peptide - 15924 15924
PKC Substrate Peptide - 17518 17518
PKC Substrate Peptide - 20634 20634
PKC Substrate Peptide - 2064523 2064523
Show more

References

Reference overviewApplicationPub Med ID
Nuclear diacylglycerol produced by phosphoinositide-specific phospholipase C is responsible for nuclear translocation of protein kinase C-alpha
Neri, L. M., et al
J Biol Chem, 273:29738-44 (1998)  1998

Kinase Assay9792687 9792687
Identification of two essential phosphorylated threonine residues in the catalytic domain of Mekk1. Indirect activation by Pak3 and protein kinase C
Siow, Y. L., et al
J Biol Chem, 272:7586-94 (1997)  1997

Kinase Assay9065412 9065412
A synthetic peptide substrate for selective assay of protein kinase C.
Yasuda, I, et al.
Biochem. Biophys. Res. Commun., 166: 1220-7 (1990)  1990

Show Abstract 		1689574 1689574

Related Products & Applications

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Kinase & Phosphatase Substrates
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Kinase & Phosphatase Substrates