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14-484 PKCα Protein, active, 10 µg

Active, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.

PKCα Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-484
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-484
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKCα Protein, active, 10 µg
OverviewRecombinant, full-length human PKCα, containing N-terminal His6 tag

PKC Lipid activator (Catalog # 20-133) is required for use.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant, full-length human PKCα, containing N-terminal His6 tag. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Gene Symbol
  • PRKCA
  • PKC-A
  • PKCA
  • MGC129901
  • AAG6
  • MGC129900
  • PRKACA
  • PKC-alpha
Protein TargetPKCα
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
SIZE: 672 amino acids; 76764 Da
SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Molecular Weight78kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-484M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-484 04053252329326

Documentation

PKCα Protein, active, 10 µg MSDS

Title

Safety Data Sheet (SDS) 

PKCα Protein, active, 10 µg Certificates of Analysis

TitleLot Number
PKC#945;, active - 33245U-M 33245U-M
PKC#945;, active - 33245U-N 33245U-N
PKC#945;, active - 33245U-P 33245U-P

FAQ

QuestionAnswer
Why is it important to shake the reaction mixture during the incubations?It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-484M PKCα Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases