Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

539677 PKCθ, His•Tag®, Human, Recombinant, S. frugiperda

PKCθ, His•Tag®, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase Cθ

539677
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
OverviewFull-length, recombinant, human PKC fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PKC is a calcium-independent PKC, which is expressed in T lymphocytes and muscle. PKC is reported to be required for T cell activation, proliferation, and cytokine production.
Catalogue Number539677
Brand Family Calbiochem®
SynonymsProtein Kinase Cθ
References
ReferencesAltman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
Villalba, M., et al. 2001. J. Immunol. 166, 5955.
Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC<sub>ε</sub> Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5
EC number2.7.1
FormLiquid
FormulationIn 150 mM NaCl, 20 mM HEPES, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% NP-40, pH 7.5.
Applications
Biological Information
Primary TargetPKCθ
Purity≥90% by SDS-PAGE
Specific Activity≥2500 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539677 0

Documentation

PKCθ, His•Tag®, Human, Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

PKCθ, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
539677

References

Reference overview
Altman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
Villalba, M., et al. 2001. J. Immunol. 166, 5955.
Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-November-2008 JSW
SynonymsProtein Kinase Cθ
DescriptionFull-length, recombinant, human PKC fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PKC is a calcium-independent PKC, which is expressed in T lymphocytes and muscle. PKC is reported to be required for T cell activation, proliferation, and cytokine production.
FormLiquid
FormulationIn 150 mM NaCl, 20 mM HEPES, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% NP-40, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
20 mM HEPES, pH 7.5, 10 mM MgCl2, 100 µM EGTA, 100 µg/ml PKCε substrate (Cat. No. 539562), 100 µM ATP, 200 µg/ml phosphatidylserine, 20 µg/ml diacylglycerol, 0.03% Triton® X-100, trace [32P]-γ-ATP, 5 µl enzyme in a total reaction volume of 50 µl; incubate at 30°C for the desired time.
EC number2.7.1
Purity≥90% by SDS-PAGE
Specific activity≥2500 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKCε Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAltman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
Villalba, M., et al. 2001. J. Immunol. 166, 5955.
Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.