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539513 PKC, Catalytic Subunit, Rat Brain

PKC, Catalytic Subunit, Rat Brain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase C-M

539513
  
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Overview

Replacement Information
Description
OverviewNative PKC catalytic subunit purified from rat brain. Prepared by trypsin digestion. Does not require Ca2+ or phosphatidylserine for its activity.

This product has been discontinued.
Catalogue Number539513
Brand Family Calbiochem®
SynonymsProtein Kinase C-M
References
ReferencesVan Renterghem, B., et al. 1994. J. Biol. Chem. 269, 24666.
Wang, L-Y., et al. 1994. J. Physiol. 475, 431.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone III-S per min at 30°C.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 15 mM DTT, 1 mM EDTA, 1 mM EGTA, 10% glycerol, pH 7.5.
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥750 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539513 0

Documentation

PKC, Catalytic Subunit, Rat Brain MSDS

Title

Safety Data Sheet (SDS) 

PKC, Catalytic Subunit, Rat Brain Certificates of Analysis

TitleLot Number
539513

References

Reference overview
Van Renterghem, B., et al. 1994. J. Biol. Chem. 269, 24666.
Wang, L-Y., et al. 1994. J. Physiol. 475, 431.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2008 RFH
SynonymsProtein Kinase C-M
DescriptionNative PKC catalytic subunit purified from rat brain. Prepared by trypsin digestion. Does not require Ca2+ or phosphatidylserine for its activity.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM Tris-HCl, 15 mM DTT, 1 mM EDTA, 1 mM EGTA, 10% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Reaction Conditions for Protein Kinase C, Catalytic Subunit, Rat Brain

Assay Solution:
20 µl 400 mM MES, pH 6.0
20 µl 10 mM EGTA
20µl 100 mM MgCl2
20 µl 5 mg/ml histone III-S (or other substrate)
40 µl H2O
20 µl column sample or 20-25 ng purified kinase
20 µl 1.0 mM ATP (100 µCi/ml)

Reaction Protocol:
1. Start the reaction by adding the ATP.
2. Incubate for 2.5 min at 30°C in an agitating water bath.
3. Stop the reaction by adding 1 ml of cold 25% trichloroacetic acid.
4. Add 10 µl of 50 mg/ml BSA per tube.
5. Vortex for 2 min at 10,000 x g. Aspirate the supernatant.
6. Repeat steps 3-5 twice using 0.5 ml trichloroacetic acid.
7. Count dry.

Terminate the reaction at various time points (e.g. 1, 2, 4, 6, 8 min) to ensure that the reaction is linear.

This protocol is meant to serve as a guideline and may need to be modified for specific applications.
Purity≥95% by SDS-PAGE
Specific activity≥750 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone III-S per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesVan Renterghem, B., et al. 1994. J. Biol. Chem. 269, 24666.
Wang, L-Y., et al. 1994. J. Physiol. 475, 431.