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521270 PDK1, His•Tag®, Human, Recombinant, S. frugiperda

PDK1, His•Tag®, Human, Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Phosphoinositide-Dependent Protein Kinase-1

521270
  
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Overview

Replacement Information
Description
OverviewFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
Catalogue Number521270
Brand Family Calbiochem®
Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
References
ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
Biondi, R.M., et al. 2000. EMBO J. 19, 979.
Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
EC number2.7.10
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥75 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
521270 0

Documentation

PDK1, His•Tag®, Human, Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

PDK1, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
521270

References

Reference overview
Scott, M.T., et al. 2002. EMBO J. 21, 6771.
Biondi, R.M., et al. 2000. EMBO J. 19, 979.
Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.

Brochure

Title
Biologics 31.1
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-August-2014 JSW
Synonyms3-Phosphoinositide-Dependent Protein Kinase-1
DescriptionFull length, recombinant, human PDK1 fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PDK1 is a serine/threonine protein kinase that is localized to the plasma membrane. PDK1 activates conventional protein kinase C (PKC) as well as PKCζ. PDK1 also phosphorylates Akt and plays a role in insulin signaling.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
EC number2.7.10
Purity≥90% by SDS-PAGE
Specific activity≥75 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1 nmol of phosphate to PDK1 substrate per min at 30°C, pH 7.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesScott, M.T., et al. 2002. EMBO J. 21, 6771.
Biondi, R.M., et al. 2000. EMBO J. 19, 979.
Alessi, D.R., et al. 1997. Curr. Biol. 7, 776.