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521220 PDGF, AB Heterodimer, Human, Recombinant, E. coli

PDGF, AB Heterodimer, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhPDGF-AB

521220
  
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Overview

Replacement Information

Key Spec Table

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.





Recombinant, human platelet-drived growth factor AB (produced by the in vitro dimerization of PDGF-A and PDGF-B monomers.) and expressed in E. coli.
Catalogue Number521220
Brand Family Calbiochem®
SynonymsrhPDGF-AB
References
ReferencesHannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
Williams, L.T. 1989. Science 243, 1564.
Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods enzym. 109, 749.
Johnson, A., et al. 1984. EMBO J. 3, 921.
Product Information
FormLyophilized
FormulationLyophilized from sterile-filtered 40% acetonitrile, 0.1% TFA.
Applications
Biological Information
Biological activityED₅₀ = 1-3 ng/ml as measured by stimulation of ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1 EU/µg PDGF-AB
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
521220 0

Documentation

PDGF, AB Heterodimer, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
521220

References

Reference overview
Hannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
Williams, L.T. 1989. Science 243, 1564.
Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods enzym. 109, 749.
Johnson, A., et al. 1984. EMBO J. 3, 921.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-May-2008 RFH
SynonymsrhPDGF-AB
DescriptionRecombinant, human platelet-drived growth factor AB (produced by the in vitro dimerization of PDGF-A and PDGF-B monomers.) and expressed in E. coli.
FormLyophilized
FormulationLyophilized from sterile-filtered 40% acetonitrile, 0.1% TFA.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: ≤1 EU/µg PDGF-AB
Biological activityED₅₀ = 1-3 ng/ml as measured by stimulation of ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts
SolubilityReconstitute to a concentration ≥10 µg/ml with sterile 4 mM HCl containing at least 0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for 3 months at -70°C.
Toxicity Standard Handling
Merck USA index14, 7525
ReferencesHannink, M., and Donoghue, D.J. 1989. Biochim. Biophys. Acta 989, 1.
Williams, L.T. 1989. Science 243, 1564.
Deuel, T.F. 1987. Annu. Rev. Cell Biol. 3, 443.
Betsholtz, C., et al. 1986. Nature 320, 695.
Raines, E.W., et al. 1985. Methods enzym. 109, 749.
Johnson, A., et al. 1984. EMBO J. 3, 921.