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SCC279 P493-6 Human Lymphoblastoid Cell Line

P493-6 human B cell line permits conditional myc expression allowing for detailed studies of proliferative mechanisms in B cell lymphoma.

SCC279
≥1X10⁶ cells/vial   
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Overview

Replacement Information
Description
Catalogue NumberSCC279
DescriptionP493-6 Human Lymphoblastoid Cell Line
Alternate Names
  • P-493-6
  • p493-6
Background InformationThe proto-oncogene c-myc is an activator of cell proliferation, promoting cell cycle progression, and is thus significant factor in many cancers. Burkitt’s lymphoma is a rare cancer of human B cells associated with Epstein-Barr virus (EBV) that is the most rapidly growing human tumor, due to transcriptional activation of c-myc by chromosomal translocation (1). Because of its highly aggressive nature, Burkitt’s lymphoma has a high mortality rate if left untreated. Cellular models of c-myc-activated lymphomas are essential for understanding mechanisms of proliferation and for developing treatments for this disease.
The P493-6 Human Lymphoblastoid Cell Line expresses c-myc under control of a tetracycline-inducible promoter (2). P493-6 cells constitutively express c-myc when cultured without tetracycline. Addition of tetracycline switches off c-myc expression and arrests cells in G0/G1 phase. P493-6 cells demonstrate the morphology and surface antigen expression typical of Burkitt lymphoma cells, including CD10 and CD38 (3).

Source:
The P493-6 cell line was established from a clone of the EREB2-5 EBV-positive lymphoblastoid cell line stably transfected with a c-myc-tet plasmid construct (2).

References:
1. Lancet. 2012; 379(9822): 1234-1244.
2. Int J Cancer. 2000; 87(6): 787-793.
3. Int J Cancer. 2001; 93(6): 810-816
References
Product Information
Applications
ApplicationP493-6 human B cell line permits conditional myc expression allowing for detailed studies of proliferative mechanisms in B cell lymphoma.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Biological Information
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Packaging Information
Material Size≥1X10⁶ cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC279 04061841689294