Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

509290 OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G418 is an aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis.

OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NMD Inhibitor I

509290
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
108321-42-2C₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Description
OverviewAminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
Catalogue Number509290
Brand Family Calbiochem®
SynonymsNMD Inhibitor I
References
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Product Information
CAS number108321-42-2
FormWhite powder
Hill FormulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Quality LevelMQ200
Applications
Biological Information
Biological activityAssay: ≥730 µg/mg
Purity≥90% by HPLC
Physicochemical Information
ContaminantsHeavy metals: ≤100 ppm
UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSCB9378500
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
509290 0

Documentation

OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

OmniPur® G418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

TitleLot Number
509290

References

Reference overview
Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-March-2020 JSW
SynonymsNMD Inhibitor I
DescriptionAminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.
FormWhite powder
Recommended reaction conditionsThe optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 mg/ml Mammalian cells: 0.1-2.0 mg/ml A multiplying cell will be affected by the presence of G418 sooner than a resting cell. It will take at least two cell generations to achieve cell death in sensitive cell lines.
CAS number108321-42-2
RTECSCB9378500
Chemical formulaC₂₀H₄₀N₄O₁₀ · 2H₂SO₄
Purity≥90% by HPLC
ContaminantsHeavy metals: ≤100 ppm
UV Absorbance (280 nm, 100 mg/ml in H₂O) ≤ 0.35
Biological activityAssay: ≥730 µg/mg
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsSolubilization in H₂O at 100 mg/ml yields a clear, colorless solution.
Toxicity Standard Handling
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.