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567715 N-SMase Inhibitor, GW4869 - CAS 6823-69-4 - Calbiochem

N-SMase Inhibitor, GW4869 - CAS 6823-69-4, is a cell-permeable, potent, specific, non-competitive inhibitor of N-SMase (IC50 = ~ 1 µM, rat brain) Does not inhibit human acid SMase even at 150 µM.

N-SMase Inhibitor, GW4869 - CAS 6823-69-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Sphingomyelinase, Neutral, Inhibitor GW4869, GW69A, GW554869A

Primary Target: N-Smase rat brain
567715
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
6823-69-4C₃₀H₂₈N₆O₂ • 2HCl
Description
Overview

This product has been discontinued.



cell-permeable, symmetrical dihydroimidazolo-amide compound that acts as a potent, specific, non-competitive inhibitor of N-SMase (neutral sphingomyelinase) [IC50 = ~ 1 µM, rat brain; Km for sphingomyelin ~13 µM]. Does not inhibit human A-SMase (acid sphingomyelinase) even at 150 µM. Weakly inhibits the activities of bovine protein phosphatase 2A and mammalian lyso-PAF PLC, while no inhibition is observed for bacterial phosphatidylcholine-specific PLC. Reported to offer complete protection against TNF-α or diamine-induced cell death in MCF7 breast cancer cells at 20 µM. Does not modify the intracellular glutathione levels or interfere with TNF-α or diamine-mediated signaling effects.
Catalogue Number567715
Brand Family Calbiochem®
SynonymsSphingomyelinase, Neutral, Inhibitor GW4869, GW69A, GW554869A
References
ReferencesMarchesini, N., et al. 2003. J. Biol. Chem. In press.
Luberto, C., et al. 2002. J. Biol. Chem. 277, 41128
Okamoto, Y., et al. 2002. FEBS Lett. 530, 140.
Product Information
CAS number6823-69-4
ATP CompetitiveN
FormPale yellow solid
Hill FormulaC₃₀H₂₈N₆O₂ • 2HCl
Chemical formulaC₃₀H₂₈N₆O₂ • 2HCl
Hygroscopic Hygroscopic
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationN-SMase Inhibitor, GW4869 - CAS 6823-69-4, is a cell-permeable, potent, specific, non-competitive inhibitor of N-SMase (IC50 = ~ 1 µM, rat brain) Does not inhibit human acid SMase even at 150 µM.
Biological Information
Primary TargetN-Smase rat brain
Primary Target IC<sub>50</sub>1 µM rat brain N-SMase (neutral sphingomyelinase); Km for sphingomyelin ~13 µM
Purity≥90% by NMR
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
567715 0

Documentation

N-SMase Inhibitor, GW4869 - CAS 6823-69-4 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

N-SMase Inhibitor, GW4869 - CAS 6823-69-4 - Calbiochem Certificates of Analysis

TitleLot Number
567715

References

Reference overview
Marchesini, N., et al. 2003. J. Biol. Chem. In press.
Luberto, C., et al. 2002. J. Biol. Chem. 277, 41128
Okamoto, Y., et al. 2002. FEBS Lett. 530, 140.