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PSHT010R5 Millicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 µm

PSHT010R5
5  Bulk packed
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberPSHT010R5
Trade Name
  • Millicell
DescriptionMillicell-24 Cell Culture Insert Plate, polycarbonate, 0.4 µm
Background InformationMillicell cell culture insert plates are a patented device designed to support cell growth and differentiation of endothelial and epithelial cell lines, including CaCo-2.

The Millicell device is available with a 0.4 µm PCF and a variety of PET membrane pore sizes. The plate is designed for maximum user convenience and incorporates features such as apical assist for easier pipetting and basolateral access. To reduce the risk of monolayer contamination, the membrane plates have feet to elevate the plate above the work surface when disassembled from the feeder tray. Tear drop-shaped receiver wells eliminate air bubbles as plates are assembled.

Millicell plate are optimized to grow and sustain high integrity cell monolayers. Cells grown on Millicell plate membranes grow better than on plastic because the cells are nourished from both the apical and basolateral sides. Cell growth and function more closely mimic in vivo. Millicell plates are designed for analysis as well as cell growth and can be used manually or with automated cell seeding, feeding and washing systems

With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a multi-well feeding tray. At the time of transport analysis, the plate is simply transferred to a multi-well transport tray for analysis.

This streamlined design enhances functionality with:

- Seed-and-feed systems

- Automated liquid handling systems and span pipettes

- Basolateral and apical access to cells

- Transepithelial electrical resistance (TEER) measurement systems
References
Product Information
Components
  • 24-well cell culture plate, single-well feeder tray, lid
HS Code3926 90 40
Automation CompatibleYes
Device Configuration24-well plate
Filter CodePCF
Maximum Operating Temperature50 °C
Number of Wells24
Quality LevelMQ400
Applications
ApplicationPolycarbonate cell culture insert with pore size of 0.4 µm used in a 24-well pate for cell attachment, cell culture, cell differentiation, drug transport & permeability.
Key Applications
  • Cell Attachment
  • Cell Culture
  • Cell Differentiation
  • Drug Transport
  • Permeability
Biological Information
MediaIsopore®
SterilitySterile
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Pore Size0.4 µm
Dimensions
Height26.2 mm
Width84.8 mm
Length128 mm
Filtration Area0.7 cm²
Filter Diameter (⌀)9.5 mm
Materials Information
Chemistry
  • Polycarbonate (PC)
Device Material
  • Polystyrene
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size5
Material PackageBulk packed
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PSHT010R5 04053252264269

Documentation

References

Reference overviewApplication
Effects of cimetidine on the biological behaviors of human gastric cancer cells
Jiang CG, Liu FR, Xu HM, Wu T, Gao J.
Zhonghua Yi Xue Za Zhi. 2006 Jul 11;86(26):1813-6  2005

Overexpression of TIMP-1 mediated by recombinant adenovirus in hepatocellular carcinoma cells inhibits proliferation and invasion in vitro
Xia D, Yan LN, Xie JG, Tong Y, Yan ML, Wang XP, Zhang MM, Zhao LY.
Hepatobiliary Pancreat Dis Int. 2006 Aug;5(3):409-15.  2005

Development of mouse embryos co-cultured with polarized or non-polarized uterine epithelial cells using sequential culture media.
Azadbakht M, Valojerdi MR, Mowla SJ.
Anim Reprod Sci. 2006 Jul 27  2005

Cell Culture
A comparison of polarized and non-polarized human endometrial monolayer culture systems on murine embryo development.
Baghaban Eslami Nejad MR, Rezazadeh Valojerdi M, Kazemi Ashtiani S.
J Exp Clin Assist Reprod. 2005 Apr 19;2(1):7.  2004

Cell Culture
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
Toimela, T et. al.,Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.
Toxicol Appl Pharmacol. 2004 Feb 15;195(1):73-82.  2004

User Guides

Title
User Guide - Millicell-24 and -96 well cell culture plates

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Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Cell Culture Inserts