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475815 Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2 - Calbiochem

Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2, is a potent inhibitor of protein phosphatase 1 (IC50 = 1.7 nM) and protein phosphatase 2A (IC50 = 40 pM).

Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: MCYST-LR

Primary Target: PP1
475815
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
101043-37-2C₄₉H₇₄N₁₀O₁₂

Products

Catalogue NumberPackaging Qty/Pack
475815-500UG Hliníkový sud 500 μg
Description
OverviewCyclic heptapeptide toxin isolated from the freshwater cyanobacteria Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Reacts with phosphatase in a two-step mechanism involving rapid binding and inactivation of the catalytic subunit followed by slower covalent interactions. Has no effect on protein kinases, making it useful for reducing the effect of contaminating phosphatase activities in protein kinase assays. A 2.5 mM (250 µg/100 µl) solution of Microcystin-LR, Microcystis aeruginosa (Cat. No. 475821) in DMSO is also available.
Catalogue Number475815
Brand Family Calbiochem®
SynonymsMCYST-LR
References
ReferencesCraig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
Cohen, P., et al. 1989. FEBS Lett. 250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.
Product Information
CAS number101043-37-2
ATP CompetitiveN
DeclarationNot available for sale outside of the United States.
FormLyophilized pale-yellow solid
Hill FormulaC₄₉H₇₄N₁₀O₁₂
Chemical formulaC₄₉H₇₄N₁₀O₁₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationMicrocystin-LR, Microcystis aeruginosa - CAS 101043-37-2, is a potent inhibitor of protein phosphatase 1 (IC50 = 1.7 nM) and protein phosphatase 2A (IC50 = 40 pM).
Biological Information
Primary TargetPP1
Primary Target IC<sub>50</sub>1.7 nM and 40 pM against PP1 and PP2A, respectively
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSGT2810000
Safety Information
R PhraseR: 26/27/28-36/37/38-43

Very toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
May cause sensitization by skin contact.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Unstable at > pH 7.7.
End use certificateY
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
475815-500UG 04055977185591

Documentation

Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Microcystin-LR, Microcystis aeruginosa - CAS 101043-37-2 - Calbiochem Certificates of Analysis

TitleLot Number
475815

References

Reference overview
Craig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
Cohen, P., et al. 1989. FEBS Lett. 250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2008 RFH
SynonymsMCYST-LR
DescriptionCyclic heptapeptide toxin isolated from the freshwater cyanobacteria, Microcystis aeruginosa. Potent inhibitor of protein phosphatase 1 (PP1) and protein phosphatase 2A (PP2A). Unlike Okadaic Acid (Cat. No. 495604), microcystin-LR is effective on both PP1 (IC50 = 1.7 nM) and PP2A (IC50 = 40 pM). Has no effect on protein kinases, making it useful for reducing contaminating phosphatase activities in protein kinase assays.
FormLyophilized pale-yellow solid
CAS number101043-37-2
RTECSGT2810000
Chemical formulaC₄₉H₇₄N₁₀O₁₂
Structure formulaStructure formula
Purity≥95% by HPLC
SolubilityDMSO (2.5 mg/ml) or Methanol (2 mg/ml). Further dilute with aqueous buffer just prior to use.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Unstable at > pH 7.7.
Toxicity Highly Toxic
ReferencesCraig, M., et al. 1996. Biochem. Cell Biol. 74, 569.
Nishiwaki, R., et al. 1994. Cancer Lett. 83, 283.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Davidson, H.W., et al. 1992. J. Cell Biol. 116, 1343.
Nishiwaki-Matsushima, R., et al. 1992. J. Cancer Res. Clin. Oncol. 118, 420.
Cohen, P., et al. 1990. Trends Biochem. Sci. 15, 98.
MacKintosh, C., et al. 1990. FEBS Lett. 264, 187.
Cohen, P., et al. 1989. FEBS Lett. 250, 596.
Carmichael, W.W. 1988. in Handbook of Natural Toxins 3: Marine Toxins and Venoms (Tu, A.T., Ed.) p. 121, Decker, New York.