Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

444805 Membrane Traffic Inhibitor, A5 - Calbiochem

The Membrane Traffic Inhibitor, A5 controls the biological activity of adaptor complex AP-1.

Membrane Traffic Inhibitor, A5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5

444805
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₁₇H₂₄N₂O • HCl

Products

Catalogue NumberPackaging Qty/Pack
444805-10MG Plastová ampulka 10 mg
Description
OverviewA cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.
Catalogue Number444805
Brand Family Calbiochem®
Synonyms1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5
References
ReferencesDuncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA 104, 6235.
Product Information
FormPale yellow solid
Hill FormulaC₁₇H₂₄N₂O • HCl
Chemical formulaC₁₇H₂₄N₂O • HCl
Hygroscopic Hygroscopic
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444805-10MG 04055977186154

Documentation

Membrane Traffic Inhibitor, A5 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Membrane Traffic Inhibitor, A5 - Calbiochem Certificates of Analysis

TitleLot Number
444805

References

Reference overview
Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA 104, 6235.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-March-2009 RFH
Synonyms1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5
DescriptionA cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.
FormPale yellow solid
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₁₇H₂₄N₂O • HCl
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityH₂O (1 mg/ml)
Storage Protect from light
-20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesDuncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA 104, 6235.