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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

MCD8MAG-48K MilliporeMILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine CD8+ biomarkers in mouse serum, plasma and cell culture samples.

MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
References
Brochures
Technical Information
Posters

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology

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Overview

Replacement Information

Special Offers

This panel is available for custom premix. Talk to your Protein Specialist today!

Key Spec Table

Analytes Available	Species Reactivity	Key Applications	Detection Methods
sCD137 sFas sFAS ligand GM-CSF Granzyme B IFNγ IL-2 IL-4 IL-5 IL-6 IL-10 IL-13 MIP-1β RANTES TNF-α	M	Mplex	Luminex xMAP

Description
Catalogue Number	MCD8MAG-48K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
Overview	The MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel enables you to focus on the therapeutic potential of Mouse CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel is the most versatile system available for Mouse CD8+ T Cell research. • MILLIPLEX MAP offers you the ability to: • Select a 15-plex premixed option or • Choose any combination of analytes from our panel of 15 analytes to design a custom kit that better meets your needs. • A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.
Background Information	CD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: < 20 Intra-assay:< 8
Panel Type	MAGNETIC Cytokines/Chemokines
Quality Level	MQ200

Applications
Application	Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple cytokine and chemokine CD8+ biomarkers in mouse serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is a 2-hr or overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well. • This kit may be used for the analysis of all or any combination of the CD8+ T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant

Biological Information
Species Reactivity	Mouse
Analytes Available	sCD137 sFas sFAS ligand GM-CSF Granzyme B IFNγ IL-2 IL-4 IL-5 IL-6 IL-10 IL-13 MIP-1β RANTES TNF-α
Cross Reactivity	There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol)..

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range	sCD137 IFNγ, IL-2, MIP-1ß, RANTES: 4.9 – 20,000 pg/mL sFas, sFas Ligand, GM-CSF: 24.4 – 100,000 pg/mL Granzyme B, IL-4, TNFα: 2.4 – 10,000 pg/mL IL-5: 3.7 – 15,000 pg/mL IL-6: 12.2 – 50,000 pg/mL IL-10: 6.1 – 25,000 pg/mL IL-13: 48.8 – 200,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MCD8MAG-48K	04053252987762

Documentation

MILLIPLEX MAP Mouse CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay MSDS

Title
Safety Data Sheet (SDS)

References

Reference overview	Pub Med ID
Virus-induced CD8+ T cells accelerate the onset of experimental autoimmune encephalomyelitis: implications for how viral infections might trigger multiple sclerosis exacerbations. Rainey-Barger, EK; Blakely, PK; Huber, AK; Segal, BM; Irani, DN Journal of neuroimmunology 259 47-54 2013 Show Abstract Viral infections can exacerbate multiple sclerosis (MS) through poorly defined mechanisms. We developed an experimental system whereby infection with an asymptomatic neurotropic alphavirus caused a transient acceleration of experimental autoimmune encephalomyelitis (EAE) without altering the expansion or differentiation of autoreactive CD4+ T cells. Instead, this effect on the clinical course of EAE depended on CD8+ T cells that neither participate in viral clearance nor induce neuropathology in infected mice without EAE. Our system should be useful to further unravel how certain viral infections trigger MS exacerbations and to understand how CD8+ T cells can exert pathogenic effects within active demyelinating lesions.	23602715

Reference overview

Pub Med ID

Brochure

Title
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
QUALITY CONTROL RANGES - Milliplex Mouse CD8%26#43; T Cell Magnetic Bead Panel
Quality Control Range Sheet- MCD8-110 and MCD8-210

Posters

Title
MILLIPLEX®MAP Cell Signaling Wall Chart

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