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48-625MAG MILLIPLEX® MAP 2-Plex Phospo/Total mTOR - Cell Signaling Multiplex Assay

The MILLIPLEX® MAP 2-Plex Phospho/Total mTOR kit detects total mTOR and phosphorylated mTOR (Ser2448).

MILLIPLEX® MAP 2-Plex Phospo/Total mTOR - Cell Signaling Multiplex Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Cell Signaling
48-625MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
mTOR (Total) mTOR (Ser2448) H, M, RMplexLuminex xMAP
Description
Catalogue Number48-625MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® MAP 2-Plex Phospo/Total mTOR - Cell Signaling Multiplex Assay
OverviewThe MILLIPLEX® MAP 2-Plex Phospho/Total mTOR magnetic bead kit has been developed for the simultaneous detection of phosphorylated mTOR (Ser2448) and total mTOR in a single well using the Luminex system. This detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.
Background InformationmTOR (mammalian target of rapamycin) is a Ser/Thr kinase belonging to the phosphatidylinositol 3-kinase-related kinase protein family. It mediates cell growth, cell proliferation, cell motility, cell survival, protein synthesis and transcription in response to growth factors and energy needs. mTOR is part of the Akt/mTOR pathway which is an important pathway that controls cellular metabolism. Dysfunction of this pathway has been implicated in various human diseases such as diabetes, obesity and cancer.

mTOR is encoded by the MTOR gene. The mTOR catalytic subunit exists in two structurally distinct complexes: mTORC1 and mTORC2. Each of the two complexes are composed of different interacting proteins and localized to different subcellular compartments which affect its activation and function. The core components of mTORC1 is composed of mTOR, regulatory-associated protein of mTOR (Raptor) and mammalian lethal with SEC13 protein 8 (MLST8). The core components of mTORC2 is composed of mTOR, rapamycin-insensitive companion of mTOR (RICTOR), MLST8, and mammalian stress-activated protein kinase interacting protein 1 (mSIN1).

The MILLIPLEX® MAP 2-Plex Phospho/Total mTOR kit contains all the components necessary to measure total mTOR and phosphorylated mTOR (Ser2448).
References
Product Information
Components
  • MILLIPLEX® MAP 2-plex Phospho/Total mTOR, Magnetic Beads (20X), 1 tube, 180 uL
  • MILLIPLEX® MAP 2-plex Phospho/Total mTOR, Biotin (20X) Detection Antibody, 1 tube, 180 uL
  • MILLIPLEX® MAP Lysis Buffer (1X), 1 bottle, 55 mL
  • MILLIPLEX® MAP Assay Buffer 2 (1X), 1 bottle, 55 mL
  • MILLIPLEX® MAP HeLa Cell Lysate: Phosphatase, 1 vial
  • MILLIPLEX® MAP HeLa Cell Lysate: Insulin, 1 vial
  • MILLIPLEX® MAP Streptavidin-Phycoerythrin (25X), 1 tube, 150 uL
  • MILLIPLEX® MAP Amplification Buffer (1X), 1 bottle, 3 mL
  • Empty mixing bottles, 3 bottles
  • Sample Plates, 96 well format, 2 plates
  • Foil Plate Sealers, 4
Detection methodLuminex xMAP
HS Code3002 15 90
ConfigurationPremixed
Panel TypeCell Signaling
Quality LevelMQ200
Applications
ApplicationThe MILLIPLEX® MAP 2-Plex Phospho/Total mTOR kit detects total mTOR and phosphorylated mTOR (Ser2448).
Key Applications
  • Multiplexing
Application Notes• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted, filtered cell lysate per well.
• This kit must be run using the provided Assay Buffer.
• The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL)
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • mTOR (Total)
  • mTOR (Ser2448)
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
48-625MAG 04055977162523

Documentation

Protocols

Title
Protocol- 48-625MAG

MILLIPLEX® MAP 2-Plex Phospo/Total mTOR - Cell Signaling Multiplex Assay MSDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
96 well Template 2 Up
96 well Template Single
Intracellular signal transduction pathways -MILLIPLEX®MAP Cell Signaling Phospho/Total 2-Plex assays
White Paper: Further considerations of antibody validation and usage.

User Guides

Title
User Guide-48625MAG