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407623 Interleukin-2, Human, Recombinant, E. coli

Interleukin-2, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhIL-2

407623
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human interleukin-2 expressed in E. coli. Has a variety of immunological functions. IL-2 is secreted by activated T cells. Acts as an autocrine agent to promote proliferation and maturation of activated T cells. Human IL-2 is active in murine cell lines. Exhibits about 60% amino acid homology with murine IL-2. Inhibits dexamethasone-induced apoptosis in T lymphocytes. Induces nitric oxide synthase.
Catalogue Number407623
Brand Family Calbiochem®
SynonymsrhIL-2
References
ReferencesMigliorati, G., et al. 1994. Pharmacol. Res. 30, 43.
Nakamura, Y., et al. 1994. Cancer Res. 54, 5757.
Karanth, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3383.
Maeda, A., et al. 1983. Biochem. Biophys. Res. Commun. 115, 1040.
Taniguchi, T., et al. 1983. Nature 302, 305.
Product Information
FormLyophilized
FormulationCarrier-free
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀= 80-500 pg/ml as measured in a cell proliferation assay with an IL-2-dependent murine cytotoxic T cell line
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤100 pg/µg IL-2
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSNM9742000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
407623 0

Documentation

Interleukin-2, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Interleukin-2, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
407623

References

Reference overview
Migliorati, G., et al. 1994. Pharmacol. Res. 30, 43.
Nakamura, Y., et al. 1994. Cancer Res. 54, 5757.
Karanth, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3383.
Maeda, A., et al. 1983. Biochem. Biophys. Res. Commun. 115, 1040.
Taniguchi, T., et al. 1983. Nature 302, 305.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 RFH
SynonymsrhIL-2
DescriptionRecombinant, human interleukin-2 expressed in E. coli. Has a variety of immunological functions. Acts as an autocrine agent to promote proliferation and maturation of activated T cells. A comparison of the amino acid sequences of human and murine IL-2 shows ~60% sequence similarity. Inhibits dexamethasone-induced apoptosis in T-lymphocytes. Induces nitric oxide synthase. Human IL-2 is active in murine cell lines. Validated for use in cell culture and bioassays.
FormLyophilized
FormulationCarrier-free
RTECSNM9742000
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤100 pg/µg IL-2
Biological activityED₅₀= 80-500 pg/ml as measured in a cell proliferation assay with an IL-2-dependent murine cytotoxic T cell line
SolubilityReconstitute to a concentration of 0.1-1 mg/ml with sterile 100 mM acetic acid. Further dilute in buffer or medium containing ≥0.1% BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
Merck USA index14, 4994
ReferencesMigliorati, G., et al. 1994. Pharmacol. Res. 30, 43.
Nakamura, Y., et al. 1994. Cancer Res. 54, 5757.
Karanth, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3383.
Maeda, A., et al. 1983. Biochem. Biophys. Res. Commun. 115, 1040.
Taniguchi, T., et al. 1983. Nature 302, 305.