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382184 Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda

Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.

Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HDAC8, Human, Recombinant

382184
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
382184-50UG Plastová ampulka 50 μg
Description
OverviewFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
Catalogue Number382184
Brand Family Calbiochem®
SynonymsHDAC8, Human, Recombinant
References
ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199
Product Information
Unit of DefinitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
FormLiquid
FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
Quality LevelMQ100
Applications
ApplicationHistone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥10 units/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
382184-50UG 04055977212846

Documentation

Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
382184

References

Reference overview
Waltregny, D., et al. 2005. FASEB J. 19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-May-2008 JSW
SynonymsHDAC8, Human, Recombinant
DescriptionFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
FormLiquid
FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 100 µM fluorescent substrate [Arg-His-Lys(Ac)-Lys(Ac)], 100 µg/ml BSA, 15 ng/ml HDAC8; incubate at 30°C for 60 min.
Purity≥90% by SDS-PAGE
Specific activity≥10 units/µg protein
Unit definitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
Buggy, J.J., et al. 2000. Biochem J. 350, 199