Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

444147 HCV NS3/4A Protease, Recombinant, E. coli

HCV NS3/4A Protease, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Hepatitis C Virus NS3/4A Protease, Recombinant, E. coli.

444147
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Recombinant, human HCV NS3/4A protease expressed in E. coli and purified by affinity chromatography using FPLC. HCV is a positive, single-stranded RNA virus of the Flaviviridae family that encodes a polyprotein precursor of about 3,000 amino acids. It is a common cause of chronic liver disease. HCV NS3/4A is an essential enzyme for viral replication. It is believed that HCV NS3/4A protease could play an important role in a treatment of hepatitis C infection.
Catalogue Number444147
Brand Family Calbiochem®
SynonymsHepatitis C Virus NS3/4A Protease, Recombinant, E. coli.
References
ReferencesDe Francesco, R., et al. 2003. Antiviral Res. 58, 1.
Lamarre, D., et al. 2003. Nature 426, 186.
Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
Alter, M. J. 1997. Hepatology 26, 62S.
Bartenschlager, et al. 1995. J. Virol. 69, 7519.
Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
Grakoui, A., et al. 1993. J. Virol. 67, 2832.
Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
Tomei, L., et al. 1993. J. Virol. 67, 4017.
Product Information
Unit of DefinitionOne unit is defined as 1 ng of purified protein.
FormLiquid
FormulationIn 500 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Applications
Biological Information
Biological activity20-200 ng is sufficient for an in vitro protease assay
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444147 0

Documentation

HCV NS3/4A Protease, Recombinant, E. coli Certificates of Analysis

TitleLot Number
444147

References

Reference overview
De Francesco, R., et al. 2003. Antiviral Res. 58, 1.
Lamarre, D., et al. 2003. Nature 426, 186.
Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
Alter, M. J. 1997. Hepatology 26, 62S.
Bartenschlager, et al. 1995. J. Virol. 69, 7519.
Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
Grakoui, A., et al. 1993. J. Virol. 67, 2832.
Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
Tomei, L., et al. 1993. J. Virol. 67, 4017.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-June-2008 RFH
SynonymsHepatitis C Virus NS3/4A Protease, Recombinant, E. coli.
DescriptionRecombinant, human HCV NS3/4A protease expressed in E. coli and purified by affinity chromatography using FPLC. HCV is a positive, single-stranded RNA virus in the Flaviviridae family that encodes a polyprotein precursor of about 3,000 amino acids. It is a common cause of chronic liver disease. HCV NS3/4A is an essential enzyme for viral replication. It is believed that HCV NS3/4A protease could play an important role in a treatment of hepatitis C patients.
FormLiquid
FormulationIn 500 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Biological activity20-200 ng is sufficient for an in vitro protease assay
Unit definitionOne unit is defined as 1 ng of purified protein.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesDe Francesco, R., et al. 2003. Antiviral Res. 58, 1.
Lamarre, D., et al. 2003. Nature 426, 186.
Kolykhalov, A. et al. 2000. J. Virol. 74, 2046.
Blight, K. J., et al. 1998. Antiviral Ther. 3, 71.
Alter, M. J. 1997. Hepatology 26, 62S.
Bartenschlager, et al. 1995. J. Virol. 69, 7519.
Lin, C., and Rice, C. M. 1995. Proc. Natl. Acad. Sci. U.S.A. 92, 7622.
Grakoui, A., et al. 1993. J. Virol. 67, 2832.
Hijikata, M., et al. 1993. Proc. Natl. Acad. Sci. U.S.A. 90, 10773.
Tomei, L., et al. 1993. J. Virol. 67, 4017.