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109217 Gramov roztok safranínu

109217
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1092170500 Plastová flaša 500 ml
1092172500 Plastová flaša 2.5 l
Description
Catalogue Number109217
DescriptionGram's safranine solution
OverviewThis ready-to-use Gram's safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear pink to red.
A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no.1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor the Gram staining method
Biological Information
Physicochemical Information
Density0.98 g/cm3 (20 °C)
Flash point49 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H226: Horľavá kvapalina a pary.
Precautionary Statement(s)P210: Uchovávajte mimo dosahu tepla, horúcich povrchov, iskier, otvoreného ohňa a iných zdrojov zapálenia. Nefajčite.
P233: Nádobu uchovávajte tesne uzavretú.
P240: Uzemnite a upevnite nádobu a plniace zariadenie.
P241: Používajte elektrické/ ventilačné/ osvetľovacie zariadenie do výbušného prostredia.
P242: Používajte neiskriace prístroje.
P243: Vykonajte opatrenia na zabránenie výbojom statickej elektriny.
Signal WordPozor
Storage class3 Vznietlivé kvapaliny
WGKWGK 1 mierne ohrozujúce vody
Disposal28
Vodné roztoky: nádoba D.
Safety Information
Categories of dangerhor?avá
Product Usage Statements
Storage and Shipping Information
StorageSkladujte pri +15°C až +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDKein Gefahrgut
Declaration (transport by air) IATA-DGRNo Dangerous Good
Declaration (transport by sea) IMDG-CodeNo Dangerous Good
Supplemental Information
Specifications
Suitability for microscopypasses test
gram-positve microorganismsblue-violet
gram-negative microorganismspink to red
Global Trade Item Number
Catalogue Number GTIN
1092170500 04022536112046
1092172500 04022536557397

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Alternative Products

Catalogue Number Description
111885 Gram-Color

Related Products

Catalogue Number Description
111885 Gram-Color

Accessories (Primary)

Catalogue Number Description
100567 Lugolov roztok
109218 Gramov roztok kryštalickej violeti
109261 Lugolov roztok (zriedeny roztok jódu a jodidu draselného)
110218 Gramov odfarbovací roztok

Accessories (Secondary)

Catalogue Number Description
100567 Lugolov roztok
109218 Gramov roztok kryštalickej violeti
109261 Lugolov roztok (zriedeny roztok jódu a jodidu draselného)
111885 Gram-Color

Related Products By: Application Facete

Categories

IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining