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508940 G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: G418, G 418

508940
  
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
108321-42-2C₂₀H₄₀N₄O₁₀ • 2H₂SO₄
Description
OverviewRecommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
Catalogue Number508940
Brand Family Calbiochem®
SynonymsG418, G 418
References
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Product Information
CAS number108321-42-2
FormLiquid
FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.
Hill FormulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
Chemical formulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
Quality LevelMQ200
Applications
Biological Information
Purity≥98% by TLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 21/22

Harmful in contact with skin and if swallowed.
S PhraseS: 24-36

Avoid contact with skin.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage -20°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
508940 0

Documentation

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

G 418 Sulfate, Sterile-Filtered Solution in 100 mM HEPES, Cell Culture Tested - CAS 108321-42-2 - Calbiochem Certificates of Analysis

TitleLot Number
508940

References

Reference overview
Ethier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-December-2014 JSW
SynonymsG418, G 418
DescriptionRecommended reaction conditions: The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 µg/ml Plant cells: 10-100 µg/ml Yeast cells: 0.5-1.0 µg/ml Mammalian cells: 0.1-2.0 µg/ml
FormLiquid
FormulationSterile-filtered solution of Cat. No. 345810 supplied at 50 mg/ml in 100 mM HEPES, pH 7.3.
Intert gas (Yes/No) Packaged under inert gas
CAS number108321-42-2
Chemical formulaC₂₀H₄₀N₄O₁₀ • 2H₂SO₄
Purity≥98% by TLC
Storage Protect from light
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 1 year at -20°C.
Toxicity Harmful
ReferencesEthier, S.P., and Taback, E. 1993. Cancer Lett. 74, 189.
Santerre, R.F., et al. 1991. Methods Mol. Biol. 7, 245.
Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor, NY.
Edwards, S.A., and Adamson, E.D. 1987. J. Cell Physiol. 133, 46.
Ernst, J.F., and Chan, R.K. 1985. J. Bacteriol. 163, 8.
Canaani, D., and Berg, P. 1982. Proc. Natl. Acad. Sci. USA 79, 5166.
Hirth, K.P., et al. 1982. Proc. Natl. Acad. Sci. USA 79, 7356.
Ursic, D., et al. 1981. Biochem. Biophys. Res. Commun. 101, 1031.
Jimenez, A., and Davies, J. 1980. Nature 287, 869.