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PMEF-NL-P1 EmbryoMax® Primary Mouse Embryonic Fibroblasts

PMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1

EmbryoMax® Primary Mouse Embryonic Fibroblasts MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
PMEF-NL-P1
1 vial  1x106 cells
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Key Spec Table

Key Applications
Cult
Description
Catalogue NumberPMEF-NL-P1
Trade Name
  • Embryomax
  • Specialty Media
DescriptionEmbryoMax® Primary Mouse Embryonic Fibroblasts
OverviewPMEF-NL-P1 are low passage neomycin resistant Primary Mouse Embryo Fibroblasts (PMEFs) isolated from FVB mouse embryos at day 13. The cells have NOT been mitotically arrested. PMEFs are the traditional feeder cells used to support the growth of undifferentiated mouse and human pluripotent stem cell cultures. Additionally, low passage primary cultures of fibroblasts have a high proliferative capacity and can be used for reprogramming. It is known that cellular reprogramming efficiency declines with each passage, presumably due to lowered proliferative capacity of PMEFs at later passages. These untreated PMEF cells are high quality, low passage (P1) mouse fibroblasts that have been successfully reprogrammed into iPS cells with high efficiency. They can be used to study the mechanisms of reprogramming or be further expanded and treated with γ-irradiation or mitomycin-C to make growth arrested feeder cells. See Murine Embryonic Stem Cell Culture Manual for complete culture and media for PMEF cells.
Alternate Names
  • EmbryoMax® Primary Mouse Embryonic Fibroblasts
Background InformationThe EmbryoMax range of PMEF cells provides researchers with a convenient solution for ES cell culture by eliminating the need for time consuming feeder cell isolation and preparation. Many embryonic stem cell culture protocols necessitate the use of primary mouse embryo fibroblast (PMEF) cells. In these protocols, ES cells are typically cultured on a monolayer of PMEF feeder cells. Feeder cells perform two important roles in stem cell culture: they secrete several important growth factors into the medium, which help maintain pluripotency, and they provide a cellular matrix for ES cells to grow.
Product Information
PresentationProvided frozen in EMD Millipore Freezing Medium (S-002-D) containing DMSO.
Quality LevelMQ100
Applications
ApplicationPMEF, Neo Resistant, Strain FVB, Not Treated, Passage 1
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • PMEFs
  • Induced Pluripotent Stem Cells
Cell Line Type
  • Primary Mouse Embryo Fibroblasts
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAfter receipt, vials should be stored at -80°C. If storing for longer than 6 months, store in the vapor phase of liquid nitrogen (make sure the cap is tight) for up to 2 years.
Packaging Information
Material Size1 vial
Material Package1x106 cells
Global Trade Item Number
Catalogue Number GTIN
PMEF-NL-P1 04053252851810