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SF006 ESGRO Complete Accutase

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

ESGRO Complete Accutase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
SF006
100 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSF006
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionESGRO Complete Accutase
OverviewESGRO Complete™ Accutase™ is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete™ Clonal Grade Medium (Cat. No. SF001-500). Accutase™ does not contain mammalian or bacterial derived products.
References
Product Information
HS Code3821 00 00
PresentationFrozen sterile filtered (0.2 micron) liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase™ enzymes in Dulbecco's PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.
Quality LevelMQ100
Applications
ApplicationThe ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.
Key Applications
  • Stem Cell Culture
Application NotesCell Detachment:

1. Thaw Accutase™ to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase™ to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase™ may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.
Packaging Information
Material Size100 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SF006 04053252503061

Documentation

Protocols

Title
Feeder-free and Serum-free ES Cell Culture using ESGRO Complete System

ESGRO Complete Accutase MSDS

Title

Safety Data Sheet (SDS) 

ESGRO Complete Accutase Certificates of Analysis

TitleLot Number
ESGRO COMPLETE™ ACCUTASE™ - 2300854 2300854

References

Reference overviewPub Med ID
Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos.
Gertsenstein M. et al.
PLoS One,  5(6)  e11260  2009

Show Abstract 		20582321 20582321

Brochure

Title
Murine Embryonic Stem Cell Culture Procedures & Protocols

Technical Info

Title
Derivation of Mouse Embryonic Stem Cells in Serum-Free and Feeder-Free Conditions
ESGRO Complete Clonal Grade Medium: Serum-Free Medium for Mouse Embryonic Stem Cell Culture
ESGRO Complete Products for Mouse Embryonic Stem Cell Culture in Serum-Free and Feeder-Free Conditions
The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

Data Sheet

Title
ESGRO COMPLETE™ ACCUTASE™