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324856 EGF, Murine Submaxillary Glands, Receptor Grade

324856
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Purity
≥98% by SDS-PAGE
Description
Overview

This product has been discontinued.



Native epidermal growth factor from mouse submaxillary glands. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Purified by size-exclusion chromatography followed by DEAE ion-exchange chromatography.

Effective biological range: 5-50 ng/ml.
Catalogue Number324856
Brand Family Calbiochem®
SynonymsEpidermal Growth Factor
References
ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
Sato, J., et al. 1987. Methods Enzymol. 146, 63.
Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.
Product Information
CAS number62229-50-9
FormLyophilized
Quality LevelMQ100
Applications
Biological Information
Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
Purity≥98% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSKB4518000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
324856 0

Documentation

EGF, Murine Submaxillary Glands, Receptor Grade MSDS

Title

Safety Data Sheet (SDS) 

EGF, Murine Submaxillary Glands, Receptor Grade Certificates of Analysis

TitleLot Number
324856

References

Reference overview
Canals, F., et al. 1992. Biochemistry 31, 4493.
Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
Sato, J., et al. 1987. Methods Enzymol. 146, 63.
Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-May-2008 RFH
SynonymsEpidermal Growth Factor
DescriptionNative epidermal growth factor from mouse submaxillary glands. Purified by size-exclusion chromatography. Exhibits mitogenic activity in a wide range of tissues of ectodermal and endodermal origin. Effective at concentrations ranging from 5-50 ng/ml.
FormLyophilized
CAS number62229-50-9
RTECSKB4518000
Purity≥98% by SDS-PAGE
Biological activitydetermined by mitogenic assay with human foreskin fibroblasts
SolubilityReconstitute in culture medium, buffer, H₂O, or 100 mM acetic acid. Wipe the vial with 70% ethanol, add solvent, and swirl gently to dissolve.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
Merck USA index14, 3527
ReferencesCanals, F., et al. 1992. Biochemistry 31, 4493.
Cohen, S. 1987. In Vitro Cell. Dev. Biol. 23, 239.
Sato, J., et al. 1987. Methods Enzymol. 146, 63.
Savage, C.R., and Cohen, S. 1972. J. Biol. Chem. 247, 7609.