Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

251765 DAR-4M AM - Calbiochem

A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM.

DAR-4M AM - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Diaminorhodamine-4M AM

251765
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₈H₃₁IN₄O₅

Products

Catalogue NumberPackaging Qty/Pack
251765-1MG Plastová ampulka 1 mg
Description
OverviewA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH.
Catalogue Number251765
Brand Family Calbiochem®
SynonymsDiaminorhodamine-4M AM
References
ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
Product Information
ATP CompetitiveN
DeclarationNot available for sale in Japan.
FormDark red solution
FormulationIn DMSO.
Hill FormulaC₂₈H₃₁IN₄O₅
Chemical formulaC₂₈H₃₁IN₄O₅
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetNitric oxide (NO) fluorescent indicator
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 23-26-36

Do not breathe fumes.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
251765-1MG 04055977217261

Documentation

DAR-4M AM - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

DAR-4M AM - Calbiochem Certificates of Analysis

TitleLot Number
251765

References

Reference overview
Kojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-November-2007 JSW
SynonymsDiaminorhodamine-4M AM
DescriptionA cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Intracellularly releases DAR-4M (Cat. No. 251760) by the action of esterases. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH above 4.0. Excitation max: ~560 nm; emission max: ~575 nm.
FormDark red solution
FormulationIn DMSO.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions
This product is provided at a concentration of ~5 mM. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol red may affect fluorescence.
Chemical formulaC₂₈H₃₁IN₄O₅
Structure formulaStructure formula
Purity≥98% by HPLC
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately after dilution, discard any unused portion.
Toxicity Irritant
ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.