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S7860 CpGenome™ Rat Unmethylated Genomic DNA Standard

S7860
5 µg per vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberS7860
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ Rat Unmethylated Genomic DNA Standard
OverviewThe CpGenome™ Rat Unmethylated Genomic DNA Standard is ideal for use as a control or standard in DNA methylation studies. This unmethylated standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase. This process results in a DNA standard which contains ≥ 95% unmethylated cytosines across the genome in both heterochromatic and euchromatic regions.
References
Product Information
Presentation5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA
Quality LevelMQ100
Applications
ApplicationUseful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
Biological Information
Species Reactivity
  • Rat
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceQuantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.
Packaging Information
Material Size5 µg per vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
S7860 04055977120165

Documentation

CpGenome™ Rat Unmethylated Genomic DNA Standard MSDS

Title

Safety Data Sheet (SDS) 

CpGenome™ Rat Unmethylated Genomic DNA Standard Certificates of Analysis

TitleLot Number
CpGenomeTM Rat Unmethylated Genomic DNA Standard 2943574
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 2885159 2885159
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 3062242 3062242
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 3202230 3202230
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 3519261 3519261
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 3677718 3677718
CpGenomeTM Rat Unmethylated Genomic DNA Standard - 3748761 3748761
CpGenomeTM Rat Unmethylated Genomic DNA Standard - Q2490103 Q2490103