Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

234590 Concanavalin A Plate, Clear Polystyrene

Concanavalin A Plate, Clear Polystyrene MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
234590
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Useful for the capture of glycoproteins or oligosaccharides containing terminal glucose or mannose residues. Suitable for colorimetric detection systems such as alkaline phosphatase or horseradish peroxidase. The physical and optical properties of the plate are not affected by the coupling chemistry. Data sheet included.
Catalogue Number234590
Brand Family Calbiochem®
References
ReferencesKobayashi, Y., et al. 1987. Immunology 60, 213.
O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.
Product Information
FormCon A covalently bound to clear polystyrene plate
Format96-well plate
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-40-42/43

Harmful by inhalation, in contact with skin and if swallowed.
Limited evidence of a carcinogenic effect.
May cause sensitization by inhalation and skin contact.
S PhraseS: 36

Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful & Carcinogenic / Teratogenic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
234590 0

Documentation

Concanavalin A Plate, Clear Polystyrene MSDS

Title

Safety Data Sheet (SDS) 

Concanavalin A Plate, Clear Polystyrene Certificates of Analysis

TitleLot Number
234590

References

Reference overview
Kobayashi, Y., et al. 1987. Immunology 60, 213.
O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-June-2008 RFH
DescriptionConcanavalin A (Con A), a lectin derived from the jack bean, has been used in a column format for the separation and purification of glycoproteins and carbohydrates based on the affinity of terminal mannose and glucose groups for Con A.2 Using a unique and proprietary surface chemistry procedure, our Con A plates (96 well) or strips (12 strips of 8 wells in a 96-well configuration) have the lectin covalently attached to the well surfaces. The physical and optical properties of the plate are unaffected by the coupling chemistry. The Con A plates and strips exhibit high affinity for terminal mannose and glucose groups and can be used to immobilize glycoproteins or carbohydrates containing these groups. The immobilized material can then be assayed with a standard ELISA system using tagged antibodies to the immobilized species.
The plates or strips are available in clear or opaque white polystyrene. The opaque white polystyrene plates or
strips can be used in fluorescent or chemiluminescent detection systems.
Con A plates can also be used as a substrate for cell growth because of the affinity of the cell surface carbohydrate groups for Con A. It is possible to do cell panning, selecting those cells containing terminal mannose or glucose groups for immobilization; however, please note that these plates are not sterile. To use them for cell growth studies, it may be necessary to sterilize them. They can be safely sterilized either by ethanol or irradiation.
FormCon A covalently bound to clear polystyrene plate
Recommended reaction conditionsSuggested Protocol 1. Rehydrate the plate or strips by filling each well with buffer at neutral pH and allow to sit for 10 min. Rinse twice with buffer. 2. Fill the wells with the glycoprotein or carbohydrate in an appropriate buffer at a concentration of 1-3. µg/ml at neutral pH. The buffer should contain 1 mM Ca2+ and 1 mM Mn2+. 3. Allow binding to proceed for 2 h at 37°C. 4. Wash 3 times with buffer to remove unbound material and extra ions. 5. To use in an immunoassay, add a tagged antibody at 1-3 µg/ml in buffer and allow to react for 2 h or less at 37°C. The time depends on the speed of the antibody-antigen reaction. Typically, either horseradish peroxidase or alkaline phosphatase is used as tags. 6. Develop color using the appropriate substrate. For a usual assay, color development with alkaline phosphatase takes about an hour and horseradish peroxidase takes about 15 min.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Harmful & Carcinogenic / Teratogenic
ReferencesKobayashi, Y., et al. 1987. Immunology 60, 213.
O'Shannessy, D.J., and Hofmann, W.L. 1987. Biotech. Appl. Biochem. 9, 488.