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HTS054M ChemiScreen™ CXCR6 Membrane Preparation

Human CXCR6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

ChemiScreen™ CXCR6 Membrane Preparation MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-5 Uni Prot Number: O00574 Entrez Gene Number: NM_006564.1
HTS054M
200 units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-5CXCR6
Description
Catalogue NumberHTS054M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
DescriptionChemiScreen™ CXCR6 Membrane Preparation
OverviewHuman CXCR6 cDNA
Background InformationThe GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001) CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005). CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Chemicon's CXCR6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR6 interactions with its ligands. The membrane preparations exhibit a Kd of 0.6 nM for [125I]-CXCL16. With 2.5 or 10 μg/well CXCR6 Membrane Prep and 0.5 nM [125I]-CXCL16, greater than 4-fold and 9-fold signal-to-background ratios are obtained, respectively.
References
Product Information
FormatMembranes
PresentationOne vial contains enough membranes for at least 200 assays (units), where one unit is the amount of membrane that will yield greater than 1000 cpm specific CXCL16-stimulated [35S]-GTPγS binding. If radioligand binding assays are desired, it is recommended to double the mass of membrane prep per well to increase signal window..

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.

Packaging method: Membrane protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80oC.
Quality LevelMQ100
Applications
ApplicationHuman CXCR6 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application NotesRadioligand binding assay, and GTPγS binding.
Biological Information
Entrez Gene Number
Gene Symbol
  • CXCR6
  • CDw186
  • BONZO
  • CXC-R6
  • CXCR-6
  • TYMSTR
  • CD186
  • STRL33
GPCR ClassA
Host CellsChem-5
Protein TargetCXCR6
Target Sub-FamilyChemokine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O00574 # Receptor for the C-X-C chemokine CXCL16. Used as a coreceptor by SIVs and by strains of HIV-2 and m-tropic HIV-1.
SIZE: 342 amino acids; 39280 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in lymphoid tissues and activated T cells.
SIMILARITY: SwissProt: O00574 ## Belongs to the G-protein coupled receptor 1 family.
Incubation ConditionsRECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C

Radioligand: [125I] CXCL16 (Perkin Elmer catalog # NEX398)

Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 4-fold signal:background with 125I-labeled CXCL16 at 0.5 nM
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSPECIFICATIONS:
1 unit = 5 μg for GTPγS binding assay
EC50 in GTPγS binding assay by CXCL16: 0.52 – 1.2 nM
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.
Packaging Information
Material Size200 units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS054M 04053252677854

Documentation

ChemiScreen™ CXCR6 Membrane Preparation MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells.
Tabata S et al.
J. Leukoc. Biol. , 77:777-86 (2005)  2004

15703197 15703197
Pathogenic role of the CXCL16-CXCR6 pathway in rheumatoid arthritis.
Nanki, Toshihiro, et al.
Arthritis Rheum., 52: 3004-14 (2005)  2004

Show Abstract 		16200580 16200580
Role for CXCR6 in recruitment of activated CD8+ lymphocytes to inflamed liver.
Sato T et al.
J. Immunol., 174:277-83 (2005)  2004

15611250 15611250
Expression cloning of the STRL33/BONZO/TYMSTR ligand reveals elements of CC, CXC, and CX3C chemokines.
Wilbanks A et al.
J. Immunol., 166:5145-5154 (2001)  2001

11290797 11290797

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